Anti-Apg7/ATG7 Antibody Picoband™

ATG7 antibody

Boster Bio Anti-Apg7/ATG7 Antibody Picoband™ catalog # PB9479. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: PB9479
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Apg7/ATG7 Antibody Picoband™

View all ATG7 Antibodies

SKU/Catalog Number

PB9479

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Apg7/ATG7 Antibody Picoband™ catalog # PB9479. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apg7/ATG7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9479)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7, different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9479 is reactive to ATG7 in Human, Mouse, Rat

Applications

PB9479 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

78 kDa

Calculated molecular weight

77.96kDa

Background of ATG7

Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ATG7 (Source: Uniprot.org, NCBI)

Gene Name

ATG7

Full Name

Ubiquitin-like modifier-activating enzyme ATG7

Weight

77.96kDa

Superfamily

ATG7 family

Alternative Names

APG7 autophagy 7-like (S. cerevisiae); APG7; APG7L; APG7-LIKE; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7; Autophagy-related protein 7; DKFZp434N0735; GSA7; hAGP7; ubiquitin activating enzyme E1-like protein; Ubiquitin-activating enzyme E1-like protein; ubiquitin-like modifier-activating enzyme ATG7 ATG7 APG7-LIKE, APG7L, GSA7 autophagy related 7 ubiquitin-like modifier-activating enzyme ATG7|APG7 autophagy 7-like|ATG12-activating enzyme E1 ATG7|hAGP7|ubiquitin-activating enzyme E1-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATG7, check out the ATG7 Infographic

ATG7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9479 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Erbin exerts a protective effect against inflammatory bowel disease by suppressing autophagic cell death

Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in the development of mouse placenta

Yang J, Sheng S, Yang Q, Li L, Qin S, Yu S, Zhang X. Oncol Lett. 2017 Nov;14(5):5333-5339. doi: 10.3892/ol.2017.6857. Epub 2017 Aug 31. Endocan silencing induces programmed cell death in hepatocarcinoma

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5 Customer Q&As for Anti-Apg7/ATG7 Antibody Picoband™

Question

We are currently using anti-Apg7/ATG7 antibody PB9479 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

The anti-Apg7/ATG7 antibody (PB9479) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-31

Question

Is a blocking peptide available for product anti-Apg7/ATG7 antibody (PB9479)?

L. Miller

Verified customer

Asked: 2019-12-12

Answer

We do provide the blocking peptide for product anti-Apg7/ATG7 antibody (PB9479). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-12

Question

Can you help my question with product PB9479, anti-Apg7/ATG7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Baker

Verified customer

Asked: 2019-11-18

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9479 anti-Apg7/ATG7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Apg7/ATG7 antibody PB9479. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-11-26

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-26

Question

Is there a BSA free version of anti-Apg7/ATG7 antibody PB9479 available?

Z. Wu

Verified customer

Asked: 2016-05-27

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Apg7/ATG7 antibody PB9479 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-05-27

Order DetailsPrice
PB9479

100μg

$370
PB9479-10ug

10μg sample (liquid)

$99
PB9479-Biotin

100 μg Biotin conjugated

$570
PB9479-Cy3

100 μg Cy3 conjugated

$570
PB9479-Dylight488

100 μg Dylight488 conjugated

$570
PB9479-Dylight550

100 μg Dylight550 conjugated

$570
PB9479-Dylight594

100 μg Dylight594 conjugated

$570
PB9479-FITC

100 μg FITC conjugated

$570
PB9479-HRP

100 μg HRP conjugated

$570
PB9479-APC

100 μg APC conjugated

$670
PB9479-PE

100 μg PE conjugated

$670
PB9479-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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PB9479
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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