Data & Images
|Product Name||Anti-APOA1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Apolipoprotein A-I(APOA1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-APOA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1247)|
|Replacement Item||This antibody may replace the following items: sc-19029|sc-23605|sc-30089|sc-376811|sc-376818|sc-58230|sc-69755|sc-80551 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human APOA1 (42-58aa YVDVLKDSGRDYVSQFE), different from the related mouse and rat sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Apolipoprotein A-I(Apo-AI/ApoA-I)|
|Molecular Weight||30778 MW|
|Protein Function||Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. .|
|Tissue Specificity||Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. .|
|Sequence Similarities||Belongs to the apolipoprotein A1/A4/E family.|
|Alternative Names||Apolipoprotein A-I;Apo-AI;ApoA-I;Apolipoprotein A1;Proapolipoprotein A-I;ProapoA-I;Truncated apolipoprotein A-I;Apolipoprotein A-I(1-242);APOA1;|
|Research Areas|||cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism| signal transduction| kits/ lysates/ other|elisa kits|cancer proteins elisa kits| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipoprotein elisa kits||
Background for Apolipoprotein A-I(Apo-AI/ApoA-I)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-APOA1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-APOA1 Antibody Images
Click the images to enlarge.
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: HT1080 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,