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SKU:PA1009
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Apoptosis Inhibitor 5 Antibody
SKU/Catalog Number PA1009
Description Rabbit IgG polyclonal antibody for Apoptosis inhibitor 5(API5) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Cite This Product Anti-Apoptosis Inhibitor 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1009)
Replacement Item This antibody may replace the following items: sc-393341|sc-101203|sc-167096|sc-167094|sc-135080|sc-374528 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5(487-504aa KYSSNLGNFNYERSLQGK)(b), different from the related mouse sequence by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name API5
Protein Name Apoptosis inhibitor 5(API-5)
Molecular Weight 59005 MW
Protein Function Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. .
Tissue Specificity Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. .
Sequence Similarities Belongs to the API5 family.
Subcellular Localization Nucleus . Cytoplasm . Mainly nuclear. Can also be cytoplasmic.
Uniprot ID Q9BZZ5
Alternative Names Apoptosis inhibitor 5;API-5;Antiapoptosis clone 11 protein;AAC-11;Cell migration-inducing gene 8 protein;Fibroblast growth factor 2-interacting factor;FIF;Protein XAGL;API5;MIG8;
Research Areas API5|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Apoptosis inhibitor 5(API-5)

Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5(API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Anti-Apoptosis Inhibitor 5 Antibody Images

Click the images to enlarge.

Anti-Apoptosis Inhibitor 5 Antibody
Anti-Apoptosis inhibitor 5 antibody, PA1009, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Placenta Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: CEM Cell Lysate
Lane 8: SMMC Cell Lysate
Lane 9: COLO320 Cell Lysate
Anti-Apoptosis Inhibitor 5 Antibody
Anti-Apoptosis inhibitor 5 antibody, PA1009, IHC(P)
IHC(P): Rat Brain Tissue
Anti-Apoptosis Inhibitor 5 Antibody
Anti-Apoptosis inhibitor 5 antibody, PA1009, ICC
ICC: HELA Cell
Anti-Apoptosis Inhibitor 5 Antibody
Anti-Apoptosis inhibitor 5 antibody, PA1009, IHC(F)
IHC(F): Rat Cardiac Muscle Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.