|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-Apoptosis Inhibitor 5 Antibody|
|Description||Rabbit IgG polyclonal antibody for Apoptosis inhibitor 5(API5) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-Apoptosis Inhibitor 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1009)|
|Replacement Item||This antibody may replace the following items: sc-393341|sc-101203|sc-167096|sc-167094|sc-135080|sc-374528 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5(487-504aa KYSSNLGNFNYERSLQGK)(b), different from the related mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Apoptosis inhibitor 5(API-5)|
|Molecular Weight||59005 MW|
|Protein Function||Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. .|
|Tissue Specificity||Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. .|
|Sequence Similarities||Belongs to the API5 family.|
|Subcellular Localization||Nucleus . Cytoplasm . Mainly nuclear. Can also be cytoplasmic.|
|Alternative Names||Apoptosis inhibitor 5;API-5;Antiapoptosis clone 11 protein;AAC-11;Cell migration-inducing gene 8 protein;Fibroblast growth factor 2-interacting factor;FIF;Protein XAGL;API5;MIG8;|
Background for Apoptosis inhibitor 5(API-5)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Apoptosis Inhibitor 5 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Apoptosis Inhibitor 5 Antibody Images
Click the images to enlarge.
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Placenta Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: CEM Cell Lysate
Lane 8: SMMC Cell Lysate
Lane 9: COLO320 Cell Lysate
IHC(P): Rat Brain Tissue
ICC: HELA Cell
IHC(F): Rat Cardiac Muscle Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,