Anti-Apoptosis repressor with CARD/NOL3 Antibody

ARC/NOL3 antibody

Boster Bio Anti-Apoptosis repressor with CARD/NOL3 Antibody catalog # PA1793. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1793
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Apoptosis repressor with CARD/NOL3 Antibody

View all ARC/NOL3 Antibodies

SKU/Catalog Number

PA1793

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Apoptosis repressor with CARD/NOL3 Antibody catalog # PA1793. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apoptosis repressor with CARD/NOL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1793)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1793 is reactive to NOL3 in Human, Mouse, Rat

Applications

PA1793 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

22.629kDa

Background of ARC/NOL3

NOL3 (Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For NOL3 (Source: Uniprot.org, NCBI)

Gene Name

NOL3

Full Name

Nucleolar protein 3

Weight

22.629kDa

Alternative Names

Apoptosis repressor with CARD; ARC; ARCFLJ35304; CARD2; Muscle-enriched cytoplasmic protein; MYC; MYP; NOL3; NOP30; NOPnucleolar protein 3; nucleolar protein 3 (apoptosis repressor with CARD domain); Nucleolar protein of 30 kDa NOL3 ARC, FCM, MYOCL1, MYP, NOP, NOP30 nucleolar protein 3 nucleolar protein 3|muscle-enriched cytoplasmic protein|nucleolar protein 3 (apoptosis repressor with CARD domain)|nucleolar protein of 30 kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NOL3, check out the NOL3 Infographic

NOL3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NOL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Apoptosis repressor with CARD/NOL3 Antibody

Question

Does anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 work on feline WB with liver?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

Our lab technicians have not validated anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-25

Question

I see that the anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-12

Question

We are currently using anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

M. Rodriguez

Verified customer

Asked: 2014-02-04

Answer

The anti-Apoptosis repressor with CARD/NOL3 antibody (PA1793) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-02-04

Question

Is a blocking peptide available for product anti-Apoptosis repressor with CARD/NOL3 antibody (PA1793)?

V. Edwards

Verified customer

Asked: 2013-01-15

Answer

We do provide the blocking peptide for product anti-Apoptosis repressor with CARD/NOL3 antibody (PA1793). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-01-15

Order DetailsPrice
PA1793

100μg

$370
PA1793-10ug

10μg sample (liquid)

$99
PA1793-Biotin

100 μg Biotin conjugated

$570
PA1793-Cy3

100 μg Cy3 conjugated

$570
PA1793-Dylight488

100 μg Dylight488 conjugated

$570
PA1793-Dylight550

100 μg Dylight550 conjugated

$570
PA1793-Dylight594

100 μg Dylight594 conjugated

$570
PA1793-FITC

100 μg FITC conjugated

$570
PA1793-HRP

100 μg HRP conjugated

$570
PA1793-APC

100 μg APC conjugated

$670
PA1793-PE

100 μg PE conjugated

$670
PA1793-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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