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SKU:PA1220
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-Aquaporin 4 Antibody
SKU/Catalog Number PA1220
Description Rabbit IgG polyclonal antibody for Aquaporin-4(AQP4) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-Aquaporin 4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1220)
Replacement Item This antibody may replace the following items: sc-20812|sc-9887|sc-9888|sc-32739|sc-390636|sc-390488 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4(172-188aa FTIFASCDSKRTDVTGS), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name AQP4
Protein Name Aquaporin-4(AQP-4)
Molecular Weight 34830 MW
Protein Function Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Tissue Specificity Brain - muscle >> heart, kidney, lung, and trachea.
Sequence Similarities Belongs to the MIP/aquaporin (TC 1.A.8) family.
Subcellular Localization Membrane; Multi-pass membrane protein.
Uniprot ID P55087
Alternative Names Aquaporin-4;AQP-4;Mercurial-insensitive water channel;MIWC;WCH4;AQP4;
Research Areas |signal transduction|metabolism|plasma membrane|channels| metabolism|types of disease|cancer|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Aquaporin-4(AQP-4)

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1.Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence(designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

Anti-Aquaporin 4 Antibody Images

Click the images to enlarge.

Anti-Aquaporin 4 Antibody
Anti-Aquaporin 4 antibody, PA1220, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: COLO320 Cell Lysate
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Publications

Chen Hm, Huang Hs, Ruan L, He Yb, Li Xj. Int J Clin Exp Med. 2014 May 15;7(5):1483-9. Ecollection 2014. Ulinastatin Attenuates Cerebral Ischemia-Reperfusion Injury In Rats.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: I have seen bands on my Western Blot even after blocking the assay with the corresponding 10X peptide (Boster Catalog# BP1220). I am concerned about the specificity of the antibody and would like to know how it has been validated. Do you have a good positive and negative control for cell lysates?
A: First of all, we suggest you use a 100X peptide. Secondly, this gene is highly expressed in brain tissue (human, mouse, rat), so use Hele, MCF-7 or COLO cell in brain tissue as your positive control. For negative control, take kidney, lung or spleen tissue. However, its expression is very low.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to aquaporin-4 antibody, aqp4 antibody