Anti-Aquaporin 5/AQP5 Antibody

Rabbit IgG polyclonal antibody for Aquaporin-5(AQP5) detection. Tested with WB in Human.

SKU PA1553
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-Aquaporin 5/AQP5 Antibody
See all AQP5 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1553
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Polyclonal antibody for AQP5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. AQP5 information: Molecular Weight: 28292 MW; Subcellular Localization: Membrane; Multi-pass membrane protein.
Cite This Product Anti-Aquaporin 5/AQP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1553)
Specificity Anti-Aquaporin 5/AQP5 Antibody (PA1553) reacts with Human AQP5, in native form and recombinant. Superfamily members of AQP5 are not reactive to PA1553.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 5(246-265aa DEDWEEQREERKKTMELTTR).
Reactivity Human

Assay Details

Our Boster Quality Guarantee for Anti-Aquaporin 5/AQP5 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Aquaporin 5/AQP5 Antibody (PA1553).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1553-1-WB-anti-aquaporin-5-antibody.jpg

Anti-Aquaporin 5 antibody, PA1553, Western blotting
Lane 1: MM453 Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: SW620 Cell Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P55064
Gene Name AQP5
Protein Name Aquaporin-5
Alternative Names Aquaporin-5;AQP-5;AQP5;
Subcellular Localization Membrane; Multi-pass membrane protein.
Molecular Weight 28292 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.
Research Areas Human

*You can search these to find other products in these research areas.
Background Aquaporin 5, also known as AQP5, is a water channel protein. The aquaporins(AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0(MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP4 in ciliary epithelium and retinal Müller cells, and AQP5 in corneal and lacrimal gland epithelia. Among the seven human aquaporins cloned to date(AQPs 0-6), genes encoding the four most closely related aquaporins(AQP0, AQP2, AQP5, and AQP6) have been mapped to chromosome band 12q13, suggesting an aquaporin family gene cluster at this locus. Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions.

Other Recommended Resources

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Publishing with Anti-Aquaporin 5/AQP5 Antibody (PA1553)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]

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In stock
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PA1553
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

Hyperosmotic stress induces cisplatin sensitivity in ovarian cancer cells by stimulating aquaporin-5 expression

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to aqp5 antibody, aquaporin 5 antibody, aquaporin-5 antibody
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