Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody

Aromatase antibody

Boster Bio Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody catalog # M00071. Tested in WB, IP applications. This antibody reacts with Human, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: M00071
Size: 100 μl
Reactive Species: Human, Rat
Host: Rabbit
Application: IP, WB

Customers Who Bought This Also Bought

Product Name

Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody

View all Aromatase Antibodies

SKU/Catalog Number

M00071

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody catalog # M00071. Tested in WB, IP applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00071)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AABB-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Aromatase

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00071 is reactive to CYP19A1 in Human, Rat

Applications

M00071 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

57.883kDa

Background of Aromatase

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CYP19A1 (Source: Uniprot.org, NCBI)

Gene Name

CYP19A1

Full Name

Aromatase

Weight

57.883kDa

Superfamily

cytochrome P450 family

Alternative Names

ARO1EC 1.14.14.1; Aromatase; AROsubfamily XIX (aromatization of androgens); CYARMGC104309; CYPXIX; cytochrome P450, family 19, subfamily A, polypeptide 1; Cytochrome P-450AROM; Estrogen synthase; microsomal monooxygenase CYP19A1 ARO, ARO1, CPV1, CYAR, CYP19, CYPXIX, P-450AROM cytochrome P450 family 19 subfamily A member 1 aromatase|cytochrome P-450AROM|cytochrome P450 19A1|cytochrome P450, family 19, subfamily A, polypeptide 1|cytochrome P450, subfamily XIX (aromatization of androgens)|estrogen synthase|estrogen synthetase|flavoprotein-linked monooxygenase|microsomal monooxygenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYP19A1, check out the CYP19A1 Infographic

CYP19A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYP19A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00071 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Electroacupuncture enhances extragonadal aromatization in ovariectomized rats

Extragonadal aromatization increases with time after ovariectomy in rats

Zhang D, Yang B, Zou W, Lu X, Xiong M, Wu L, Wang J, Gao J, Xu S, Zou T. Biomed Res Int. 2013;2013:301540. Doi: 10.1155/2013/301540. Epub 2012 Dec 26. Estradiol Synthesis And Release In Cultured Female Rat Bone Marrow Stem Cells.

Have you used Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-Aromatase CYP19A1 Rabbit Monoclonal Antibody

Question

Is there a BSA free version of anti-Aromatase Rabbit Monoclonal antibody M00071 available?

A. Krishna

Verified customer

Asked: 2020-02-18

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Aromatase Rabbit Monoclonal antibody M00071 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-18

Question

Is a blocking peptide available for product anti-Aromatase Rabbit Monoclonal antibody (M00071)?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

We do provide the blocking peptide for product anti-Aromatase Rabbit Monoclonal antibody (M00071). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-12

Question

We have been able to see staining in rat chorionic villus. Any tips? Is anti-Aromatase Rabbit Monoclonal antibody supposed to stain chorionic villus positively?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

From literature chorionic villus does express CYP19A1. From Uniprot.org, CYP19A1 is expressed in chorionic villus, placenta, ovary placenta, among other tissues. Regarding which tissues have CYP19A1 expression, here are a few articles citing expression in various tissues:
Ovary, and Placenta, Pubmed ID: 15489334
Placenta, Pubmed ID: 2541021, 2691883, 2973313, 3018730, 3964273

Boster Scientific Support

Answered: 2020-01-09

Question

Please see the WB image, lot number and protocol we used for placenta using anti-Aromatase Rabbit Monoclonal antibody M00071. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-06

Question

I have a question about product M00071, anti-Aromatase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Miller

Verified customer

Asked: 2019-11-26

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00071 anti-Aromatase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-26

Question

I was wanting to use your anti-Aromatase Rabbit Monoclonal antibody for WB for rat placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat placenta identification?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

You can see on the product datasheet, M00071 anti-Aromatase Rabbit Monoclonal antibody has been validated for IP, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-03

Question

Would anti-Aromatase Rabbit Monoclonal antibody M00071 work for WB with placenta?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

According to the expression profile of placenta, CYP19A1 is highly expressed in placenta. So, it is likely that anti-Aromatase Rabbit Monoclonal antibody M00071 will work for WB with placenta.

Boster Scientific Support

Answered: 2019-08-22

Question

I am looking for to test anti-Aromatase Rabbit Monoclonal antibody M00071 on rat placenta for research purposes, then I may be interested in using anti-Aromatase Rabbit Monoclonal antibody M00071 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

The products we sell, including anti-Aromatase Rabbit Monoclonal antibody M00071, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-19

Question

My team were well pleased with the WB result of your anti-Aromatase Rabbit Monoclonal antibody. However we have seen positive staining in chorionic villus endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is CYP19A1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-16

Answer

Based on literature, chorionic villus does express CYP19A1. Generally CYP19A1 expresses in endoplasmic reticulum membrane. Regarding which tissues have CYP19A1 expression, here are a few articles citing expression in various tissues:
Ovary, and Placenta, Pubmed ID: 15489334
Placenta, Pubmed ID: 2541021, 2691883, 2973313, 3018730, 3964273

Boster Scientific Support

Answered: 2019-07-16

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Aromatase Rabbit Monoclonal antibody M00071. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-12-19

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-19

Question

Is this M00071 anti-Aromatase Rabbit Monoclonal antibody reactive to the isotypes of CYP19A1?

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

The immunogen of M00071 anti-Aromatase Rabbit Monoclonal antibody is A synthesized peptide derived from human Aromatase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-05-29

Question

I see that the anti-Aromatase Rabbit Monoclonal antibody M00071 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-09-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-09-07

Question

We have tried in the past anti-Aromatase Rabbit Monoclonal antibody for WB on chorionic villus in a previous experiment. I am using human, and We want to use the antibody for IP next. I would like examining chorionic villus as well as ovary placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

N. Walker

Verified customer

Asked: 2017-04-18

Answer

I looked at the website and datasheets of our anti-Aromatase Rabbit Monoclonal antibody and I see that M00071 has been tested on human in both WB and IP. Thus M00071 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-04-18

Question

We are currently using anti-Aromatase Rabbit Monoclonal antibody M00071 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on canine tissues as well?

C. Huang

Verified customer

Asked: 2016-10-21

Answer

The anti-Aromatase Rabbit Monoclonal antibody (M00071) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-10-21

Question

Will M00071 anti-Aromatase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Wu

Verified customer

Asked: 2013-10-07

Answer

You can see on the product datasheet, M00071 anti-Aromatase Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-10-07

Order DetailsPrice
M00071

100μL

$370
M00071-30ul

30μL sample

$99
M00071-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M00071
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.