Anti-ASPP2 TP53BP2 Antibody

53BP2 antibody

Boster Bio Anti-ASPP2 TP53BP2 Antibody (Catalog # A02609). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A02609
Size: 100ug
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-ASPP2 TP53BP2 Antibody

View all 53BP2 Antibodies

SKU/Catalog Number

A02609

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ASPP2 TP53BP2 Antibody (Catalog # A02609). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-ASPP2 TP53BP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02609)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02609 is reactive to TP53BP2 in Human

Applications

A02609 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

125.616kDa

Background of 53BP2

ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21.  Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,500 - 1:10,000
IHC: User optimized
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

Gene/Protein Information For TP53BP2 (Source: Uniprot.org, NCBI)

Gene Name

TP53BP2

Full Name

Apoptosis-stimulating of p53 protein 2

Weight

125.616kDa

Superfamily

ASPP family

Alternative Names

apoptosis-stimulating protein of p53, 2; ASPP2P53BP2,53BP2apoptosis-stimulating of p53 protein 2; Bbp; Bcl2-binding protein; p53-binding protein 2; p53BP2; PPP1R13A; Renal carcinoma antigen NY-REN-51; tumor protein p53 binding protein, 2; tumor protein p53-binding protein, 2; Tumor suppressor p53-binding protein 2 TP53BP2 53BP2, ASPP2, BBP, P53BP2, PPP1R13A tumor protein p53 binding protein 2 apoptosis-stimulating of p53 protein 2|BCL2-binding protein|apoptosis-stimulating protein of p53, 2|renal carcinoma antigen NY-REN-51|tumor suppressor p53-binding protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TP53BP2, check out the TP53BP2 Infographic

TP53BP2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TP53BP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-ASPP2 TP53BP2 Antibody

Question

We want to test anti-ASPP2 antibody A02609 on human renal cell carcinoma for research purposes, then I may be interested in using anti-ASPP2 antibody A02609 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The products we sell, including anti-ASPP2 antibody A02609, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-31

Question

I have a question about product A02609, anti-ASPP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02609 anti-ASPP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-18

Question

Is there a BSA free version of anti-ASPP2 antibody A02609 available?

W. Banerjee

Verified customer

Asked: 2015-12-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ASPP2 antibody A02609 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-12-03

Order DetailsPrice
A02609

100 μg

$599

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

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