|Sample Size:||30ug for $99, contact us for details|
|Application:||Flow Cytometry, IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-ATF1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Cyclic AMP-dependent transcription factor ATF-1(ATF1) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Rat.|
|Cite This Product||Anti-ATF1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9130)|
|Replacement Item||This antibody may replace the following items: sc-13147|sc-186|sc-186-X|sc-241|sc-241-X|sc-243|sc-243-X|sc-25785|sc-25785-X|sc-270|sc-270-X|sc-28673|sc-28673-X|sc-32551|sc-328|sc-328-G|sc-365525|sc-365525-X|sc-374227|sc-374227-X|sc-377154|sc-377154-X|sc-398555|sc-53172|sc-7978|sc-7978-R|sc-7978-X|sc-9010 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
|Application||Flow Cytometry, IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human ATF1 recombinant protein (Position: M1-V271). Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclic AMP-dependent transcription factor ATF-1|
|Molecular Weight||29233 MW|
|Protein Function||This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation. .|
|Sequence Similarities||Belongs to the bZIP family. ATF subfamily.|
|Alternative Names||Cyclic AMP-dependent transcription factor ATF-1;cAMP-dependent transcription factor ATF-1;Activating transcription factor 1;Protein TREB36;ATF1;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper||
Background for Cyclic AMP-dependent transcription factor ATF-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ATF1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ATF1 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti ATF1 (PB9130) at 0.5ug/ml
WB: Recombinant Human ATF1 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti ATF1 (PB9130) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
IHC(P): Human Intestinal Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,