|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-ATF2 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Cyclic AMP-dependent transcription factor ATF-2(ATF2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-ATF2 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9131)|
|Replacement Item||This antibody may replace the following items: sc-101635|sc-101636|sc-101637|sc-101638|sc-13147|sc-135686|sc-187|sc-187-X|sc-242|sc-242-X|sc-32551|sc-328|sc-328-G|sc-398555|sc-52941|sc-6233|sc-6233-X|sc-7982|sc-7982-R|sc-7982-X|sc-8398|sc-8398-X|sc-9010 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human ATF2 recombinant protein (Position: E93-E450). Human ATF2 shares 99% amino acid (aa) sequence identity with both mouse and rat ATF2.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclic AMP-dependent transcription factor ATF-2|
|Molecular Weight||54537 MW|
|Protein Function||Transcriptional activator which regulates the transcription of various genes, including those involved in anti- apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. .|
|Tissue Specificity||Ubiquitously expressed, with more abundant expression in the brain.|
|Sequence Similarities||Belongs to the bZIP family. ATF subfamily.|
|Subcellular Localization||Nucleus. Cytoplasm. Mitochondrion outer membrane. Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co- localizes with the MRN complex in the IR-induced foci (IRIF).|
|Alternative Names||Cyclic AMP-dependent transcription factor ATF-2;cAMP-dependent transcription factor ATF-2;18.104.22.168 ;Activating transcription factor 2;Cyclic AMP-responsive element-binding protein 2;CREB-2;cAMP-responsive element-binding protein 2;HB16;Histone acetyltransferase ATF2;cAMP response element-binding protein CRE-BP1;ATF2;CREB2, CREBP1;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| immunology|innate immunity|tlr signaling||
Background for Cyclic AMP-dependent transcription factor ATF-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ATF2 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ATF2 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti ATF2 (PB9131) at 0.5ug/ml
WB: Recombinant Human ATF2 Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Mouse Brain Tissue
IHC(P): Rat Brain Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,