|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-ATF6 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Cyclic AMP-dependent transcription factor ATF-6 alpha(ATF6) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-ATF6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1011-1)|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ATF6(599-614aa NINENVINGQDYEVMM), different from the related mouse and rat sequences by one amino acid.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Anti- ATF6 antibody, PA1011-1, Western blotting
All lanes: Anti ATF6 (PA1011-1) at 0.5ug/ml
WB: A549 Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 90KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||Cyclic AMP-dependent transcription factor ATF-6 alpha|
|Alternative Names||Cyclic AMP-dependent transcription factor ATF-6 alpha;cAMP-dependent transcription factor ATF-6 alpha;Activating transcription factor 6 alpha;ATF6-alpha;Processed cyclic AMP-dependent transcription factor ATF-6 alpha;ATF6;|
|Subcellular Localization||Endoplasmic reticulum membrane; Single-pass type II membrane protein.|
|Molecular Weight||74585 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. .|
|Background||ATF6, a member of the leucine zipper protein family, is an endoplasmic reticulum(ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules. ATF6 gene is mapped to chromosome 1q23.3. ATF6 can constitutively induce the promoter of glucose-regulated protein(grp) genes through activation of the endoplasmic reticulum(ER) stress element(ERSE).|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,