Product Info Summary
SKU: | A00956-1 |
---|---|
Size: | 100ul |
Reactive Species: | Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ATP1 alpha1/Na+K+ ATPase1 Antibody
View all Sodium Potassium ATPase Alpha 1 Antibodies
SKU/Catalog Number
A00956-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATP1 alpha1/Na+K+ ATPase1 Antibody (Catalog# A00956-1). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP1 alpha1/Na+K+ ATPase1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00956-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A7
Isotype
Rabbit IgG
Immunogen
A peptide derived from rat ATP1 alpha1/Na+K+ ATPase1. Immunogen sequence location: 15-64
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00956-1 is reactive to ATP1A1 in Rat
Applications
A00956-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
112.896kDa
Background of Sodium Potassium ATPase Alpha 1
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HeLa cells, using ATP1 alpha1/Na+K+ ATPase1 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using ATP1 alpha1/Na+K+ ATPase1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ATP1A1 (Source: Uniprot.org, NCBI)
Gene Name
ATP1A1
Full Name
Sodium/potassium-transporting ATPase subunit alpha-1
Weight
112.896kDa
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
ATPase, Na+/K+ transporting, alpha 1 polypeptide; EC 3.6.3; EC 3.6.3.9; K-ATPase alpha-1 subunit; K-ATPase catalytic subunit alpha-A protein; MGC3285; MGC51750; Na; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase 1; sodium pump 1; Sodium pump subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide ATP1A1 CMT2DD, HOMGSMR2 ATPase Na+/K+ transporting subunit alpha 1 sodium/potassium-transporting ATPase subunit alpha-1|ATPase, Na+/K+ transporting, alpha 1 polypeptide|Na(+)/K(+) ATPase alpha-1 subunit|Na+/K+ ATPase 1|Na, K-ATPase, alpha-A catalytic polypeptide|Na,K-ATPase alpha-1 subunit|Na,K-ATPase catalytic subunit alpha-A protein|sodium pump subunit alpha-1|sodium-potassium ATPase catalytic subunit alpha-1|sodium-potassium-ATPase, alpha 1 polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP1A1, check out the ATP1A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP1 alpha1/Na+K+ ATPase1 Antibody (A00956-1)
Hello CJ!
A00956-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li H,Ma Z,Zhai Y,Lv C,Yuan P,Zhu F,Wei L,Li Q,Qi X.Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR α.Front Pharmacol.2020 Aug 14;11:1255.doi:10.
Species: Rat
A00956-1 usage in article: APP:WB, SAMPLE:MYOCARDIAL AND HEPATIC TISSUE, DILUTION:NA
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