Product Info Summary
SKU: | A02064 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-ATP1A2 Antibody
SKU/Catalog Number
A02064
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATP1A2 Antibody (Catalog# A02064). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP1A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02064)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A7
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ATP1A2. Immunogen sequence location: 971 - 1020
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02064 is reactive to ATP1A2 in Human, Monkey, Mouse, Rat
Applications
A02064 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
112.265kDa
Background of ATP1A2
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from COS7 cells, HepG2 cells, and Jurkat cells, using ATP1A2 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ATP1A2 (Source: Uniprot.org, NCBI)
Gene Name
ATP1A2
Full Name
Sodium/potassium-transporting ATPase subunit alpha-2
Weight
112.265kDa
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
Sodium/potassium-transporting ATPase subunit alpha-2 ATP1A2 FHM2, MHP2 ATPase Na+/K+ transporting subunit alpha 2 sodium/potassium-transporting ATPase subunit alpha-2|ATPase Na+/K+ transporting alpha 2 polypeptide|Na(+)/K(+) ATPase alpha-2 subunit|Na+/K+ ATPase, alpha-A(+) catalytic polypeptide|Na+/K+ ATPase, alpha-B polypeptide|sodium pump subunit alpha-2|sodium-potassium ATPase catalytic subunit alpha-2|sodium/potassium-transporting ATPase alpha-2 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP1A2, check out the ATP1A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP1A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP1A2 Antibody (A02064)
Hello CJ!
No publications found for A02064
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Recommended Resources
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-ATP1A2 Antibody
Question
We are currently using anti-ATP1A2 antibody A02064 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The anti-ATP1A2 antibody (A02064) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-24
Question
Is there a BSA free version of anti-ATP1A2 antibody A02064 available?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ATP1A2 antibody A02064 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-03
Question
I am looking for to test anti-ATP1A2 antibody A02064 on rat ovary for research purposes, then I may be interested in using anti-ATP1A2 antibody A02064 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
The products we sell, including anti-ATP1A2 antibody A02064, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-ATP1A2 antibody A02064. Let me know if you need anything else.
G. Moore
Verified customer
Asked: 2015-01-09
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-09