SKU A31840
Size 100 ul
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Applications WB


Product Name Anti-ATP5MD Antibody
SKU/Catalog Number A31840
Storage & Handling -20°C
Size 100 ul
Description Anti-ATP5MD Antibody tested in WB, reactive to human|mouse|rat
Cite This Product Anti-ATP5MD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31840)
Host Rabbit
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human USMG5
Reactivity Human, Mouse, Rat

Assay Details

Images And Assay Conditions

/a/3/a31840 wb anti atp5md antibody figure 1.jpg

Figure 1. Western blot analysis of ATP5MD using anti-ATP5MD antibody (A31840).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: Human heart

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5MD antigen affinity purified polyclonal antibody (Catalog # A31840) at 0.2-1 ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5MD. The expected band size for ATP5MD is at 6.4KD.

Target Info

Protein Target Info (Source:

Uniprot Id Q96IX5
Gene Name ATP5MD
Protein Name Up-regulated during skeletal muscle growth protein 5
Alternative Names Up-regulated during skeletal muscle growth protein 5;Diabetes-associated protein in insulin-sensitive tissues;HCV F-transactivated protein 2;USMG5;DAPIT, HCVFTP2;PD04912;
Subcellular Localization Mitochondrion membrane ; Single-pass membrane protein .
Molecular Weight 6458 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.


Protein Function Plays a critical role in maintaining the ATP synthase population in mitochondria.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
1 Unit $299

USD $299

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