| SKU | A31840 |
|---|---|
| Size | 100 ul |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Host | Rabbit |
| Applications | WB |
Overview
| Product Name | Anti-ATP5MD Antibody |
|---|---|
| SKU/Catalog Number | A31840 |
| Storage & Handling | -20°C |
| Size | 100 ul |
| Description | Anti-ATP5MD Antibody tested in WB, reactive to human|mouse|rat |
| Cite This Product | Anti-ATP5MD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31840) |
| Host | Rabbit |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human USMG5 |
| Reactivity | Human, Mouse, Rat |
Assay Details
Images And Assay Conditions
Figure 1. Western blot analysis of ATP5MD using anti-ATP5MD antibody (A31840).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: Human heart
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5MD antigen affinity purified polyclonal antibody (Catalog # A31840) at 0.2-1 ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5MD. The expected band size for ATP5MD is at 6.4KD.
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | Q96IX5 |
|---|---|
| Gene Name | ATP5MD |
| Protein Name | Up-regulated during skeletal muscle growth protein 5 |
| Alternative Names | Up-regulated during skeletal muscle growth protein 5;Diabetes-associated protein in insulin-sensitive tissues;HCV F-transactivated protein 2;USMG5;DAPIT, HCVFTP2;PD04912; |
| Subcellular Localization | Mitochondrion membrane ; Single-pass membrane protein . |
| Molecular Weight | 6458 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Plays a critical role in maintaining the ATP synthase population in mitochondria. |
|---|
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