SKU A31840
Size 100 ul
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Applications WB

Overview

Product Name Anti-ATP5MD Antibody
SKU/Catalog Number A31840
Storage & Handling -20°C
Size 100 ul
Description Anti-ATP5MD Antibody tested in WB, reactive to human|mouse|rat
Cite This Product Anti-ATP5MD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31840)
Host Rabbit
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human USMG5
Reactivity Human, Mouse, Rat

Assay Details

Images And Assay Conditions

/a/3/a31840 wb anti atp5md antibody figure 1.jpg

Figure 1. Western blot analysis of ATP5MD using anti-ATP5MD antibody (A31840).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: Human heart

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5MD antigen affinity purified polyclonal antibody (Catalog # A31840) at 0.2-1 ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5MD. The expected band size for ATP5MD is at 6.4KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q96IX5
Gene Name ATP5MD
Protein Name Up-regulated during skeletal muscle growth protein 5
Alternative Names Up-regulated during skeletal muscle growth protein 5;Diabetes-associated protein in insulin-sensitive tissues;HCV F-transactivated protein 2;USMG5;DAPIT, HCVFTP2;PD04912;
Subcellular Localization Mitochondrion membrane ; Single-pass membrane protein .
Molecular Weight 6458 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Plays a critical role in maintaining the ATP synthase population in mitochondria.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Order Product (A31840)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
1 Unit $299

USD $299

Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for A31840