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SKU:PA2262
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-ATP7b Antibody
SKU/Catalog Number PA2262
Description Rabbit IgG polyclonal antibody for Copper-transporting ATPase 2(ATP7B) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-ATP7b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2262)
Replacement Item This antibody may replace the following items: sc-32446|sc-33826|sc-32445|sc-373964 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human ATP7b(1451-1465aa DKWSLLLNGRDEEQYI), different from the related mouse and rat sequences by three amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ATP7B
Protein Name Copper-transporting ATPase 2
Molecular Weight 157263 MW
Protein Function Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
Tissue Specificity Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
Sequence Similarities Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
Subcellular Localization Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels.
Uniprot ID P35670
Alternative Names Copper-transporting ATPase 2;3.6.3.54;Copper pump 2;Wilson disease-associated protein;WND/140 kDa;ATP7B;PWD, WC1, WND;
Research Areas |signal transduction|metabolism|vitamins / minerals| metabolism|pathways and processes|cofactors, vitamins / minerals|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Copper-transporting ATPase 2

ATPase, Cu++ transporting, beta polypeptide(Wilson disease) protein, also called ATP7B, is an ATPase that transports copper. This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least two putative copper-binding sites. ATP7B is mapped to 13q14.3. This protein functions as a monomer, exporting copper out of the cells. When copper levels are in excess, ATP7B redistributes to a vesicular compartment near the biliary canalicular membranes for elimination of excess copper into bile, and it is transported along liver cell microtubules via interaction with the p62 dynactin subunit.

Anti-ATP7b Antibody Images

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Anti-ATP7b Antibody
Anti-ATP7b antibody, PA2262, IHC(P)
IHC(P): Mouse Liver Tissue
Anti-ATP7b Antibody
Anti-ATP7b antibody, PA2262, IHC(P)
IHC(P): Rat Liver Tissue
Anti-ATP7b Antibody
Anti-ATP7b antibody, PA2262, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-ATP7b Antibody
Anti-ATP7b antibody, PA2262, All Western blotting
All lanes: Anti-ATP7B(PA2262) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: NEURO Whole Cell Lysate at 40ug
Predicted bind size: 157KD
Observed bind size: 157KD
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Publications

Wu Dl, Yi Hx, Sui Fy, Jiang Xh, Jiang Xm, Zhao Yy. World J Gastroenterol. 2006 Dec 21;12(47):7695-8. Expression Of Atp7B In Human Gastric Cardiac Carcinomas In Comparison With Distal Gastric Carcinomas.
Zhang Y, Zhang Q, Fan Z, Sun J, Liu X, Cheng L, Li A, Xu J. Pathol Oncol Res. 2015 Apr;21(2):389-97. Doi: 10.1007/S12253-014-9831-5. Epub 2014 Aug 8. A Chinese Herbal Formula, Chang-Wei-Qin, Synergistically Enhances Antitumor Effect Of Oxaliplatin.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.