Product Info Summary
SKU: | A00203-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF |
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Product info
Product Name
Anti-Transcriptional regulator ATRX ATRX Antibody
SKU/Catalog Number
A00203-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Transcriptional regulator ATRX ATRX Antibody (Catalog # A00203-1). Tested in IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcriptional regulator ATRX ATRX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00203-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human ATRX.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00203-1 is reactive to ATRX in Human, Mouse
Applications
A00203-1 is guaranteed for IF Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
282.586kDa
Background of ATRX
Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according () is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts. May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis.
Picketts D.J., Hum. Mol. Genet. 5:1899-1907(1996).
Villard L., Genomics 43:149-155(1997).
Kitano T., Mol. Biol. Evol. 20:1281-1289(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of A549 cells, using ATRX antibody A00203-1.
Protein Target Info & Infographic
Gene/Protein Information For ATRX (Source: Uniprot.org, NCBI)
Gene Name
ATRX
Full Name
Transcriptional regulator ATRX
Weight
282.586kDa
Superfamily
SNF2/RAD54 helicase family
Alternative Names
alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae)homolog); alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S.cerevisiae); alpha thalassemia/mental retardation syndrome X-linked; ATP-dependent helicase ATRX; ATR2; DNA dependent ATPase and helicase; EC 3.6.1; EC 3.6.4.12; helicase 2, X-linked; Juberg-Marsidi syndrome; MGC2094; MRXHF1; RAD54 homolog; RAD54L; SFM1; SHS; transcriptional regulator ATRX; XH2RAD54; X-linked helicase II; X-linked nuclear protein; XNPZNF-HX; Zinc finger helicase; Znf-HX ATRX JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX ATRX chromatin remodeler transcriptional regulator ATRX|ATP-dependent helicase ATRX|X-linked helicase II|X-linked nuclear protein|alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATRX, check out the ATRX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATRX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcriptional regulator ATRX ATRX Antibody (A00203-1)
Hello CJ!
A00203-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Co-occurrence of ATRX and IDH Mutations Identify Subgroup of Glioma Patients for Better Survival. Trivedi Trupti,Panchal Kinjal,Bhalala Neha,Jyotishi Charmi,Trivedi Priti,Panchal Harsha,Modi Nikhi
Species: Human
A00203-1 usage in article: APP:IHC, SAMPLE:GLIOMA TISSUE, DILUTION:1:100
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
15 Customer Q&As for Anti-Transcriptional regulator ATRX ATRX Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-ATRX antibody A00203-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-01
Question
I have a question about product A00203-1, anti-ATRX antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00203-1 anti-ATRX antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-19
Question
I have attached the WB image, lot number and protocol we used for frontal cortex using anti-ATRX antibody A00203-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-15
Question
We have been able to see staining in mouse cervix carcinoma. Are there any suggestions? Is anti-ATRX antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
According to literature cervix carcinoma does express ATRX. According to Uniprot.org, ATRX is expressed in frontal cortex, brain, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ATRX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15772651
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-01-06
Question
Would A00203-1 anti-ATRX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
It shows on the product datasheet, A00203-1 anti-ATRX antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-13
Question
you antibody using your anti-ATRX antibody for x-linked studies. Has this antibody been tested with western blotting on a549 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We appreciate your inquiry. This A00203-1 anti-ATRX antibody is validated on a549 cells. It is guaranteed to work for IF in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-28
Question
Is a blocking peptide available for product anti-ATRX antibody (A00203-1)?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
We do provide the blocking peptide for product anti-ATRX antibody (A00203-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-22
Question
Is there a BSA free version of anti-ATRX antibody A00203-1 available?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ATRX antibody A00203-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-03
Question
Is this A00203-1 anti-ATRX antibody reactive to the isotypes of ATRX?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The immunogen of A00203-1 anti-ATRX antibody is Synthesized peptide derived from N-terminal of human ATRX. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-03
Question
Our lab were content with the WB result of your anti-ATRX antibody. However we have seen positive staining in frontal cortex nucleus. chromosome using this antibody. Is that expected? Could you tell me where is ATRX supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to literature, frontal cortex does express ATRX. Generally ATRX expresses in nucleus. chromosome, telomere. nucleus, pml. Regarding which tissues have ATRX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15772651
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-08-26
Question
I was wanting to use your anti-ATRX antibody for IF for human frontal cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human frontal cortex identification?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
It shows on the product datasheet, A00203-1 anti-ATRX antibody has been tested for IF on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-13
Question
We are currently using anti-ATRX antibody A00203-1 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
M. Roberts
Verified customer
Asked: 2018-04-17
Answer
The anti-ATRX antibody (A00203-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-17
Question
I am interested in to test anti-ATRX antibody A00203-1 on human frontal cortex for research purposes, then I may be interested in using anti-ATRX antibody A00203-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Miller
Verified customer
Asked: 2016-09-30
Answer
The products we sell, including anti-ATRX antibody A00203-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-09-30
Question
I see that the anti-ATRX antibody A00203-1 works with IF, what is the protocol used to produce the result images on the product page?
A. Collins
Verified customer
Asked: 2016-06-22
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-06-22
Question
Does anti-ATRX antibody A00203-1 work for IF with frontal cortex?
O. Jha
Verified customer
Asked: 2014-10-01
Answer
According to the expression profile of frontal cortex, ATRX is highly expressed in frontal cortex. So, it is likely that anti-ATRX antibody A00203-1 will work for IF with frontal cortex.
Boster Scientific Support
Answered: 2014-10-01