|Sample Size:||30ug for $99, contact us for details|
|Application:||Flow Cytometry, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-ATX2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Ataxin-2(ATXN2) detection. Tested with WB, IHC-F, ICC, FCM in Human;Mouse;Rat.|
|Cite This Product||Anti-ATX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2098)|
|Replacement Item||This antibody may replace the following items: sc-34835|sc-365643|sc-28912|sc-34833|sc-6886|sc-166752|sc-377140 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||Flow Cytometry, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ATX2(1293-1313aa TTAHFPYMTHPSVQAHHQQQL), identical to the related mouse sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||140283 MW|
|Protein Function||Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane. .|
|Tissue Specificity||Expressed in the brain, heart, liver, skeletal muscle, pancreas and placenta. Isoform 1 is predominant in the brain and spinal cord. Isoform 4 is more abundant in the cerebellum. In the brain, broadly expressed in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus. .|
|Subcellular Localization||Cytoplasm .|
|Alternative Names||Ataxin-2;Spinocerebellar ataxia type 2 protein;Trinucleotide repeat-containing gene 13 protein;ATXN2;ATX2, SCA2, TNRC13;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|translation|regulation| neuroscience|neurology process|neurodegenerative disease| cell biology|other antibodies||
Background for Ataxin-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ATX2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ATX2 Antibody Images
Click the images to enlarge.
Lane 1: PANC Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: HELA Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,