|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-B MyB Antibody|
|Description||Rabbit IgG polyclonal antibody for Myb-related protein B(MYBL2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-B MyB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1928)|
|Replacement Item||This antibody may replace the following items: sc-31166|sc-31164|sc-20735|sc-6930|sc-122|sc-122-G|sc-122-X|sc-122|sc-122-G|sc-122-X|sc-20735|sc-30195|sc-31164|sc-31166|sc-5608|sc-6930|sc-7099 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human B MyB(1-17aa MSRRTRCEDLDELHYQD), identical to the related mouse sequence and different from the related rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Myb-related protein B|
|Molecular Weight||78764 MW|
|Protein Function||Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene. .|
|Sequence Similarities||Contains 3 HTH myb-type DNA-binding domains.|
|Alternative Names||Myb-related protein B;B-Myb;Myb-like protein 2;MYBL2;BMYB;|
|Research Areas|||cell biology|cell cycle|cell differentiation| epigenetics and nuclear signaling|transcription|other factors|transcription factors| cancer|oncoproteins/suppressors|oncoproteins|chip'ing antibodies||
Background for Myb-related protein B
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-B MyB Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-B MyB Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human MYBL2, 46.8KD (162aa tag+ M1-L245)
Lane 1: Recombinant Human MYBL2 Protein 10ng
Lane 2: Recombinant Human MYBL2 Protein 5ng
Lane 3: Recombinant Human MYBL2 Protein 2.5ng
IHC(P): Rat Brain Tissue
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: MCF-7 Cell Lysate
ICC: A549 Cell
IHC(P): Human Tonsil Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,