Anti-B Raf/BRAF Antibody Picoband™

B-Raf antibody

Boster Bio Anti-B Raf/BRAF Antibody Picoband™ catalog # PB9164. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9164
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, WB

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Product Name

Anti-B Raf/BRAF Antibody Picoband™

View all B-Raf Antibodies

SKU/Catalog Number

PB9164

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-B Raf/BRAF Antibody Picoband™ catalog # PB9164. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-B Raf/BRAF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9164)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9164 is reactive to BRAF in Human, Mouse, Rat

Applications

PB9164 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

84 kDa

Calculated molecular weight

84.437kDa

Background of B-Raf

BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For BRAF (Source: Uniprot.org, NCBI)

Gene Name

BRAF

Full Name

Serine/threonine-protein kinase B-raf

Weight

84.437kDa

Superfamily

protein kinase superfamily

Alternative Names

94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109 BRAF B-RAF1, B-raf1, NS7, RAFB1, BRAF B-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase B-raf|94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|B-Raf serine/threonine-protein|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BRAF, check out the BRAF Infographic

BRAF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BRAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9164 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Feng,S.,Wang,S.,Sun,S.,Su,H.,& Zhang,L.(2021).Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.Molecular Medicine Reports,23,294.h
Species: Rat
PB9164 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:1000

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1 Customer Q&As for Anti-B Raf/BRAF Antibody Picoband™

Question

We are currently using anti-B Raf/BRAF antibody PB9164 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

T. Li

Verified customer

Asked: 2018-02-12

Answer

The anti-B Raf/BRAF antibody (PB9164) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-12

Order DetailsPrice
PB9164

100μg

$370
PB9164-10ug

10μg sample (liquid)

$99
PB9164-Biotin

100 μg Biotin conjugated

$570
PB9164-Cy3

100 μg Cy3 conjugated

$570
PB9164-Dylight488

100 μg Dylight488 conjugated

$570
PB9164-Dylight550

100 μg Dylight550 conjugated

$570
PB9164-Dylight594

100 μg Dylight594 conjugated

$570
PB9164-FITC

100 μg FITC conjugated

$570
PB9164-HRP

100 μg HRP conjugated

$570
PB9164-APC

100 μg APC conjugated

$670
PB9164-PE

100 μg PE conjugated

$670
PB9164-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9164
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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