Data & Images
|Product Name||Anti-TNFRSF13C/BAFFR Antibody|
|Description||Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 13C(TNFRSF13C) detection. Tested with WB in Human.|
|Cite This Product||Anti-TNFRSF13C/BAFFR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1391)|
|Replacement Item||This antibody may replace the following items: sc-242365|sc-242366|sc-242367|sc-398915 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human BAFF Receptor(1-15aa MRRGPRSLRGRDAPA).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tumor necrosis factor receptor superfamily member 13C|
|Molecular Weight||18864 MW|
|Protein Function||B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. .|
|Tissue Specificity||Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.|
|Subcellular Localization||Membrane ; Single-pass type III membrane protein .|
|Alternative Names||Tumor necrosis factor receptor superfamily member 13C;B-cell-activating factor receptor;BAFF receptor;BAFF-R;BLyS receptor 3;CD268;TNFRSF13C;BAFFR, BR3;|
|Research Areas|||signal transduction|growth factors/hormones|tnf||
Background for Tumor necrosis factor receptor superfamily member 13C
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TNFRSF13C/BAFFR Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TNFRSF13C/BAFFR Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Human BAFFR Protein 10ng
Lane 2: Recombinant Human BAFFR Protein 5ng
Lane 3: Recombinant Human BAFFR Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,