Anti-BAK/BAK1 Antibody Picoband™

BAK antibody

Boster Bio Anti-BAK/BAK1 Antibody Picoband™ catalog # PB9484. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PB9484
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-BAK/BAK1 Antibody Picoband™

View all BAK Antibodies

SKU/Catalog Number

PB9484

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BAK/BAK1 Antibody Picoband™ catalog # PB9484. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BAK/BAK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9484)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9484 is reactive to BAK1 in Human, Mouse, Rat

Applications

PB9484 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of BAK

BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For BAK1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16611

Gene ID

578

Gene Name

BAK1

Full Name

Bcl-2 homologous antagonist/killer

Weight

23.409kDa

Superfamily

Bcl-2 family

Alternative Names

Apoptosis regulator BAK; BAK; BAK1; BAK-LIKE; BAKMGC3887; BCL2-antagonist/killer 1; BCL2L7; bcl2-L-7; BCL2L7bcl-2 homologous antagonist/killer; BCL2-like 7 protein; Bcl-2-like protein 7; CDN1; MGC117255; pro-apoptotic protein BAK BAK1 BAK, BAK-LIKE, BCL2L7, CDN1 BCL2 antagonist/killer 1 bcl-2 homologous antagonist/killer|BCL2-like 7 protein|apoptosis regulator BAK|bcl-2-like protein 7|bcl2-L-7|pro-apoptotic protein BAK

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BAK1, check out the BAK1 Infographic

BAK1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BAK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9484 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Minicircle-oriP-IFN?: A Novel Targeted Gene Therapeutic System for EBV Positive Human Nasopharyngeal Carcinoma

BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells

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4 Customer Q&As for Anti-BAK/BAK1 Antibody Picoband™

Question

Is a blocking peptide available for product anti-BAK/BAK1 antibody (PB9484)?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

We do provide the blocking peptide for product anti-BAK/BAK1 antibody (PB9484). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-11

Question

I see that the anti-BAK/BAK1 antibody PB9484 works with WB, what is the protocol used to produce the result images on the product page?

A. Edwards

Verified customer

Asked: 2018-07-30

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-30

Question

We are currently using anti-BAK/BAK1 antibody PB9484 for human tissue, and we are happy with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

The anti-BAK/BAK1 antibody (PB9484) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-10

Question

Does PB9484 anti-BAK/BAK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Brown

Verified customer

Asked: 2014-08-19

Answer

It shows on the product datasheet, PB9484 anti-BAK/BAK1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-08-19

Size

Total: $315

SKU:PB9484

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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