|Ig Isotype||Mouse IgG1|
|Product Name||Anti-BAP1 Antibody (Monoclonal, 3C11)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for BAP1, ubiquitin carboxy-terminal hydrolase (BAP1) detection. Tested with WB in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-BAP1 Antibody (Monoclonal, 3C11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1002)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Recombinant full-length BAP1.|
Assay Dilutions Overview
Western blot, 0.5μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
Images And Assay Conditions
Anti-BAP1 antibody (monoclonal), MA1002, Western blotting
Lane: MCF-7 Cell Lysate
Protein Target Info (Source: Uniprot.org)
|Protein Name||Ubiquitin carboxyl-terminal hydrolase BAP1|
|Tissue Specificity||BAP1: Highly expressed in testis, placenta andovary. Expressed in breast.|
|Alternative Names||Ubiquitin carboxyl-terminal hydrolase BAP1;220.127.116.11;BRCA1-associated protein 1;Bap1;|
|Subcellular Localization||Cytoplasm . Nucleus . Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O. .|
|Molecular Weight||80310 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor (By similarity). .|
|Research Areas||Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Deubiquitination, Epigenetics And Nuclear Signaling, Ubiquitin & Ubiquitin Like Modifiers
*You can search these to find other products in these research areas.
|Background||BAP1, also known as BRCA1-associated protein-1, contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 is a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1 is expressed as a 4-kb mRNA in all human tissues, and mapped to 3p21.3.|
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