radio
SKU MA1002
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number 3C11
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-BAP1 Antibody (Monoclonal, 3C11)
SKU/Catalog Number MA1002
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for BAP1, ubiquitin carboxy-terminal hydrolase (BAP1) detection. Tested with WB in Human. No cross reactivity with other proteins.
Cite This Product Anti-BAP1 Antibody (Monoclonal, 3C11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1002)
Host Mouse
Immunogen Recombinant full-length BAP1.
Reactivity Human

Assay Details

Assay Dilutions Overview

Western blot, 0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

/antibody/ma1002 1 WB anti bap1 antibody monoclonal 3c11.jpg

Anti-BAP1 antibody (monoclonal), MA1002, Western blotting
Lane: MCF-7 Cell Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id D3ZHS6
Gene Name Bap1
Protein Name Ubiquitin carboxyl-terminal hydrolase BAP1
Tissue Specificity BAP1: Highly expressed in testis, placenta andovary. Expressed in breast.
Alternative Names Ubiquitin carboxyl-terminal hydrolase BAP1;3.4.19.12;BRCA1-associated protein 1;Bap1;
Subcellular Localization Cytoplasm . Nucleus . Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O. .
Molecular Weight 80310 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor (By similarity). .
Background BAP1, also known as BRCA1-associated protein-1, contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 is a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1 is expressed as a 4-kb mRNA in all human tissues, and mapped to 3p21.3.

Order Product (MA1002)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240
Ships in 5-7 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What is the sample type, loading amount and antibody dilution for the WB image displayed on your datasheet?
A: The sample type of BAP1 is human, loading amount is 30 µg and antibody dilution is 0.5 µg/mL.