Data & Images
|Product Name||Anti-BAP1 Antibody (Monoclonal, 3C11)|
|Description||Mouse IgG monoclonal antibody for BAP1, ubiquitin carboxy-terminal hydrolase (BAP1) detection. Tested with WB in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-BAP1 Antibody (Monoclonal, 3C11) 3C11 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1002)|
|Replacement Item||This antibody may replace the following items: sc-100326|sc-101109|sc-11523|sc-11524|sc-11525|sc-13576|sc-25663|sc-28236|sc-28383|sc-48386|sc-66257|sc-66261|sc-66263|sc-8132|sc-8133 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
|Immunogen||Recombinant full-length BAP1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Ubiquitin carboxyl-terminal hydrolase BAP1|
|Gene Full Name||Ubiquitin carboxyl-terminal hydrolase BAP1|
|Protein Function||Deubiquitinating enzyme that plays a key role inchromatin by mediating deubiquitination of histone H2A and HCFC1.Catalytic component of the PR-DUB complex, a complex thatspecifically mediates deubiquitination of histone H2Amonoubiquitinated at 'Lys-119' (H2AK119ub1). Does notdeubiquitinate monoubiquitinated histone H2B. Acts as a regulatorof cell growth by mediating deubiquitination of HCFC1 N-terminaland C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linkedpolyubiquitin chains. Deubiquitination of HCFC1 does not lead toincrease stability of HCFC1. Interferes with the BRCA1 and BARD1heterodimer activity by inhibiting their ability to mediateubiquitination and autoubiquitination. It however does not mediatedeubiquitination of BRCA1 and BARD1. Able to mediateautodeubiquitination via intramolecular interactions to couteractmonoubiquitination at the nuclear localization signal (NLS),thereby protecting it from cytoplasmic sequestration. Acts as atumor suppressor (By similarity)..|
|Tissue Specificity||BAP1: Highly expressed in testis, placenta andovary. Expressed in breast.|
|Sequence Similarities||Belongs to the peptidase C12 family. BAP1 subfamily.|
|Subcellular Localization||Cytoplasm.Nucleus. Mainly nuclear. Bindsto chromatin. Localizes to the cytoplasm when monoubiquitinated bythe E2/E3 hybrid ubiquitin-protein ligase UBE2O..|
|Uniprot ID||Bap1: D3ZHS6
|Alternative Names||Ubiquitin carboxyl-terminal hydrolase BAP1; 220.127.116.11; BRCA1-associated protein 1; Bap1;|
|Research Areas||cell biology|cell cycle|cell cycle inhibitors|epigenetics and nuclear signaling|cancer|ubiquitin & ubiquitin like modifiers|deubiquitination||
Background for Ubiquitin carboxyl-terminal hydrolase BAP1
At Boster we strive to provide the best Anti-BAP1 Antibody (Monoclonal, 3C11) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Application||Concentration*||Tested Species||Predicted Species||Antigen Retrieval|
Other applications have not been tested.
Optimal dilutions should be determined by end users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-BAP1 Antibody (Monoclonal, 3C11) Images
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Lane: MCF-7 Cell Lysate