Data & Images
|Product Name||Anti-BAP1 Antibody (Monoclonal, 3C11)|
|Description||Mouse IgG monoclonal antibody for BAP1, ubiquitin carboxy-terminal hydrolase (BAP1) detection. Tested with WB in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-BAP1 Antibody (Monoclonal, 3C11) 3C11 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1002)|
|Replacement Item||This antibody may replace the following items: sc-8133|sc-28236|sc-48386|sc-28383|sc-8132|sc-13576 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant full-length BAP1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Ubiquitin carboxyl-terminal hydrolase BAP1|
|Molecular Weight||80310 MW|
|Protein Function||Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor (By similarity). .|
|Tissue Specificity||BAP1: Highly expressed in testis, placenta andovary. Expressed in breast.|
|Sequence Similarities||Belongs to the peptidase C12 family. BAP1 subfamily.|
|Subcellular Localization||Cytoplasm . Nucleus . Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O. .|
|Alternative Names||Ubiquitin carboxyl-terminal hydrolase BAP1;188.8.131.52;BRCA1-associated protein 1;Bap1;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors| epigenetics and nuclear signaling| cancer|ubiquitin & ubiquitin like modifiers|deubiquitination||
Background for Ubiquitin carboxyl-terminal hydrolase BAP1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-BAP1 Antibody (Monoclonal, 3C11) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-BAP1 Antibody (Monoclonal, 3C11) Images
Click the images to enlarge.
Lane: MCF-7 Cell Lysate