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SKU:MA1002
Clonality:Monoclonal
Clone Number:3C11
Application:WB
Price: $200.00
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Overview

Product Name Anti-BAP1 Antibody (Monoclonal, 3C11)
Description Mouse IgG monoclonal antibody for BAP1, ubiquitin carboxy-terminal hydrolase (BAP1) detection. Tested with WB in Human. No cross reactivity with other proteins.
Cite This Product Anti-BAP1 Antibody (Monoclonal, 3C11) 3C11 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1002)
Replacement Item This antibody may replace the following items: sc-100326|sc-101109|sc-11523|sc-11524|sc-11525|sc-13576|sc-25663|sc-28236|sc-28383|sc-48386|sc-66257|sc-66261|sc-66263|sc-8132|sc-8133 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
Immunogen Recombinant full-length BAP1.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number 3C11
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Bap1
Protein Name Ubiquitin carboxyl-terminal hydrolase BAP1
Gene Full Name Ubiquitin carboxyl-terminal hydrolase BAP1
Protein Function Deubiquitinating enzyme that plays a key role inchromatin by mediating deubiquitination of histone H2A and HCFC1.Catalytic component of the PR-DUB complex, a complex thatspecifically mediates deubiquitination of histone H2Amonoubiquitinated at 'Lys-119' (H2AK119ub1). Does notdeubiquitinate monoubiquitinated histone H2B. Acts as a regulatorof cell growth by mediating deubiquitination of HCFC1 N-terminaland C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linkedpolyubiquitin chains. Deubiquitination of HCFC1 does not lead toincrease stability of HCFC1. Interferes with the BRCA1 and BARD1heterodimer activity by inhibiting their ability to mediateubiquitination and autoubiquitination. It however does not mediatedeubiquitination of BRCA1 and BARD1. Able to mediateautodeubiquitination via intramolecular interactions to couteractmonoubiquitination at the nuclear localization signal (NLS),thereby protecting it from cytoplasmic sequestration. Acts as atumor suppressor (By similarity)..
Tissue Specificity BAP1: Highly expressed in testis, placenta andovary. Expressed in breast.
Sequence Similarities Belongs to the peptidase C12 family. BAP1 subfamily.
Subcellular Localization Cytoplasm.Nucleus. Mainly nuclear. Bindsto chromatin. Localizes to the cytoplasm when monoubiquitinated bythe E2/E3 hybrid ubiquitin-protein ligase UBE2O..
Uniprot ID Bap1: D3ZHS6
Alternative Names Ubiquitin carboxyl-terminal hydrolase BAP1; 3.4.19.12; BRCA1-associated protein 1; Bap1;
Research Areas cell biology|cell cycle|cell cycle inhibitors|epigenetics and nuclear signaling|cancer|ubiquitin & ubiquitin like modifiers|deubiquitination|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Ubiquitin carboxyl-terminal hydrolase BAP1

BAP1, also known as BRCA1-associated protein-1, contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 is a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1 is expressed as a 4-kb mRNA in all human tissues, and mapped to 3p21.3.

Anti-BAP1 Antibody (Monoclonal, 3C11) Images

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Anti-BAP1 Antibody (Monoclonal, 3C11)
Anti-BAP1 antibody (monoclonal), MA1002, Western blotting
Lane: MCF-7 Cell Lysate
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What is the sample type, loading amount and antibody dilution for the WB image displayed on your datasheet?
A: The sample type of BAP1 is human, loading amount is 30 µg and antibody dilution is 0.5 µg/mL.