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SKU:PA1013-1
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-Bax Antibody
SKU/Catalog Number PA1013-1
Description Rabbit IgG polyclonal antibody for Apoptosis regulator BAX(BAX) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-Bax Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1013-1)
Replacement Item This antibody may replace the following items: sc-6236|sc-526|sc-7480|sc-80658|sc-65532|sc-493|sc-70408|sc-70405|sc-23959|sc-70407|sc-20067|sc-526-G|sc-526-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Bax(19-33aa IMKTGALLLQGFIQD), different from the related mouse and rat sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name BAX
Protein Name Apoptosis regulator BAX
Molecular Weight 21184 MW
Protein Function Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. .
Tissue Specificity Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. .
Sequence Similarities Belongs to the Bcl-2 family.
Subcellular Localization Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Colocalizes with 14- 3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
Uniprot ID Q07812
Alternative Names Apoptosis regulator BAX;Bcl-2-like protein 4;Bcl2-L-4;BAX;BCL2L4;
Research Areas |cell biology|apoptosis|intracellular|associated proteins| cell biology|bcl2 family| cancer|invasion/microenvironment|bcl 2 family| kits/ lysates/ other|elisa kits|apoptosis marker and proteins elisa kits| metabolism|pathways and processes|metabolism processes|cell death|apoptotic markers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Apoptosis regulator BAX

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Anti-Bax Antibody Images

Click the images to enlarge.

Anti-Bax Antibody
Anti-Bax antibody, PA1013-1, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: A549 Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 9: Human Placenta Tissue Lysate
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Publications

Chang L, Guo F, Lv Y, Wang Y, Huo B, Wang L, Liu W. Mol Biol Rep. 2013 Jan;40(1):669-73. Doi: 10.1007/S11033-012-2106-X. Epub 2012 Oct 11. The Inhibitory Effects Of Endostar Combined With Chemotherapy On Human Esophageal Squamous Cell Carcinoma Xe...
Zhao X, Chen Q, Li Y, Tang H, Liu W, Yang X. Eur J Pharm Biopharm. 2015 Jun;93:27-36. Doi: 10.1016/J.Ejpb.2015.03.003. Epub 2015 Mar 11. Doxorubicin And Curcumin Co-Delivery By Lipid Nanoparticles For Enhanced Treatment Of Diethylnitrosamine-Induc...
Lin S, Lin K, Li W, Zhou X, Huang T. Endocrine. 2010 Jun;37(3):460-6. Doi: 10.1007/S12020-010-9337-1. Epub 2010 Apr 21. Maternal Diabetes Increases Apoptosis In Mice Oocytes, Not 2-Cell Embryos.
Yan X, Wu L, Li B, Meng X, Dai H, Zheng Y, Fu J. Cardiovasc Toxicol. 2015 Jul 3. [Epub Ahead Of Print] Cyanidin-3-O-Glucoside Induces Apoptosis And Inhibits Migration Of Tumor Necrosis Factor-??-Treated Rat Aortic Smooth Muscle Cells.
Du F, Ding Y, Zou J, Li Z, Tian J, She R, Wang D, Wang H, Lv D, Chang L. Plos One. 2015 May 22;10(5):E0127047. Doi: 10.1371/Journal.Pone.0127047. Ecollection 2015. Morphology And Molecular Mechanisms Of Hepatic Injury In Rats Under Simulated Weigh...
Bu Tl, Jia Yd, Lin Jx, Mi Yl, Zhang Cq. J Zhejiang Univ Sci B. 2012 Apr;13(4):318-26. Doi: 10.1631/Jzus.B1100318. Alleviative Effect Of Quercetin On Germ Cells Intoxicated By 3-Methyl-4-Nitrophenol From Diesel Exhaust Particles.
Li Tt, Zhang Jf, Fei Sj, Zhu Sp, Zhu Jz, Qiao X, Liu Zb. Acta Pharmacol Sin. 2014 Feb;35(2):185-94. Doi: 10.1038/Aps.2013.140. Epub 2013 Dec 23. Glutamate Microinjection Into The Hypothalamic Paraventricular Nucleus Attenuates Ulcerative Colitis I...
Wang Y, Wang S, Cui W, He J, Wang Z, Yang X. Neural Regen Res. 2013 Aug 5;8(22):2021-9. Doi: 10.3969/J.Issn.1673-5374.2013.22.001. Olive Leaf Extract Inhibits Lead Poisoning-Induced Brain Injury.
Wei Q, He W, Lu Y, Yao J, Cao X. Oncol Lett. 2012 Sep;4(3):438-442. Epub 2012 Jun 7. Effect Of The Tumor Suppressor Gene Ing4 On The Proliferation Of Mcf-7 Human Breast Cancer Cells.
Zhou Yx, Qiu Yq, Xu Lq, Guo J, Li Lj. Afr J Tradit Complement Altern Med. 2012 Apr 2;9(3):405-11. Ecollection 2012. Xiao-Chai-Hu Tang In Treating Model Mice With D-Galactosamine-Induced Liver Injury.
Song Y, Zhang G, Zhu X, Pang Z, Xing F, Quan S. Oncol Lett. 2012 Sep;4(3):419-422. Epub 2012 Jun 19. Overexpression Of The Hydatidiform Mole-Related Gene F10 Inhibits Apoptosis In A549 Cells Through Downregulation Of Bcl2-Associated X Protein And ...
Sun Y, Xu Hj, Zhao Yx, Wang Lz, Sun Lr, Wang Z, Sun Xf. Evid Based Complement Alternat Med. 2013;2013:690164. Doi: 10.1155/2013/690164. Epub 2013 Mar 19. Crocin Exhibits Antitumor Effects On Human Leukemia Hl-60 Cells In Vitro And In Vivo.
Shuai H, Zhang J, Zhang J, Xie J, Zhang M, Yu Y, Zhang L. Endocr Res. 2011;36(1):25-34. Doi: 10.3109/07435800.2010.534753. Erythropoietin Protects Pancreatic ??-Cell Line Nit-1 Cells Against Cytokine-Induced Apoptosis Via Phosphatidylinositol 3-Kin...
Chen J, Huang Rb. Neurosciences (Riyadh). 2009 Oct;14(4):343-8. Protective Effect Of Yulangsan Polysaccharide On Focal Cerebral Ischemia/Reperfusion Injury In Rats And Its Underlying Mechanism.
Liu W, Zheng Q, Wang Y, Han X, Yuan L, Zhao M. Neurochem Res. 2015 Jan;40(1):70-80. Doi: 10.1007/S11064-014-1467-Z. Epub 2014 Nov 5. Transplantation Of Olfactory Ensheathing Cells Attenuates Acute Carbon Monoxide Poisoning-Induced Brain Damages In...
Yuan F, Dong P, Wang X, Fu X, Dai M, Zhang W. Exp Toxicol Pathol. 2013 Nov;65(7-8):1011-8. Doi: 10.1016/J.Etp.2013.02.004. Epub 2013 Mar 19. Toxicological Effects Of Cigarette Smoke On Ana-1 Macrophages In Vitro.
Luo Q, Li Z, Yan J, Zhu F, Xu Rj, Cai Yz. J Med Food. 2009 Aug;12(4):695-703. Doi: 10.1089/Jmf.2008.1232. Lycium Barbarum Polysaccharides Induce Apoptosis In Human Prostate Cancer Cells And Inhibits Prostate Cancer Growth In A Xenograft Mouse Mode...
Wu Cy, Tang Zh, Jiang L, Li Xf, Jiang Zs, Liu Ls. Mol Cell Biochem. 2012 Jan;359(1-2):347-58. Doi: 10.1007/S11010-011-1028-6. Epub 2011 Aug 17. Pcsk9 Sirna Inhibits Huvec Apoptosis Induced By Ox-Ldl Via Bcl/Bax-Caspase9-Caspase3 Pathway.
Rasul A, Yu B, Zhong L, Khan M, Yang H, Ma T. Oncol Rep. 2012 May;27(5):1481-7. Doi: 10.3892/Or.2012.1694. Epub 2012 Feb 21. Cytotoxic Effect Of Evodiamine In Sgc-7901 Human Gastric Adenocarcinoma Cells Via Simultaneous Induction Of Ap...
Li Y, Gu Wj, Liu Hl. Dig Dis Sci. 2012 Aug;57(8):2072-8. Doi: 10.1007/S10620-012-2132-5. Epub 2012 May 10. Induction Of Pancreatic Cancer Cell Apoptosis And Enhancement Of Gemcitabine Sensitivity By Rap80 Sirna.
Wang Y, Liu X, Zhang D, Chen J, Liu S, Berk M. Bmc Med. 2013 Feb 8;11:32. Doi: 10.1186/1741-7015-11-32. The Effects Of Apoptosis Vulnerability Markers On The Myocardium In Depression After Myocardial Infarction.
Gu Wj, Liu Hl. Anticancer Drugs. 2013 Jul;24(6):566-76. Doi: 10.1097/Cad.0B013E3283608Bc5. Induction Of Pancreatic Cancer Cell Apoptosis, Invasion, Migration, And Enhancement Of Chemotherapy Sensitivity Of Gemcitabine, 5-Fu, And Oxaliplatin By Hnr...
Wu K, Liu Y, Lv Y, Cui L, Li W, Chen J, Liang Nc, Li L. Oncol Rep. 2013 Jun;29(6):2101-8. Doi: 10.3892/Or.2013.2375. Epub 2013 Apr 3. Ent-11??-Hydroxy-15-Oxo-Kaur-16-En-19-Oic-Acid Induces Apoptosis And Cell Cycle Arrest In Cne-2Z Nasopharyngeal Ca...
Qin R, Shen H, Cao Y, Fang Y, Li H, Chen Q, Xu W. Plos One. 2013 Oct 1;8(10):E76486. Doi: 10.1371/Journal.Pone.0076486. Ecollection 2013. Tetrandrine Induces Mitochondria-Mediated Apoptosis In Human Gastric Cancer Bgc-823 Cells.
Wei T, Tian W, Liu F, Xie G. Biochem Biophys Res Commun. 2014 May 16;447(4):666-71. Doi: 10.1016/J.Bbrc.2014.04.074. Epub 2014 Apr 19. Protective Effects Of Exogenous ??-Hydroxybutyrate On Paraquat Toxicity In Rat Kidney.
Wang Y, Zhang H, Chai F, Liu X, Berk M,,,,. Bmc Psychiatry. 2014 Dec 4;14:349. Doi: 10.1186/S12888-014-0349-X. The Effects Of Escitalopram On Myocardial Apoptosis And The Expression Of Bax And Bcl-2 During Myocardial Ischemia/Reperfusion In A Mode...
Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.
Han N, Yu L, Song Z, Luo L, Wu Y. Mol Med Rep. 2015 Jul;12(1):1098-106. Doi: 10.3892/Mmr.2015.3540. Epub 2015 Mar 24. Agmatine Protects M??ller Cells From High-Concentration Glucose-Induced Cell Damage Via N-Methyl-D-Aspartic Acid Receptor Inhibition.
Sun J, Zhang H, Guan L, Zhou H, Sun M. Int J Clin Exp Med. 2015 Jan 15;8(1):358-67. Ecollection 2015. Alpha-Lipoic Acid Attenuates Trinitrobenzene Sulfonic Acid-Induced Ulcerative Colitis In Mice.
Rasul A, Ding C, Li X, Khan M, Yi F, Ali M, Ma T. Apoptosis. 2012 Oct;17(10):1104-19. Doi: 10.1007/S10495-012-0742-1. Dracorhodin Perchlorate Inhibits Pi3K/Akt And Nf-??b Activation, Up-Regulates The Expression Of P53, And Enhances Apoptosis.
Yin W, Li B, Li X, Yu F, Cai Q, Zhang Z, Wang J, Zhang J, Zhou R, Cheng M, Gao H. J Cardiovasc Pharmacol. 2015 Jan;65(1):13-21. Doi: 10.1097/Fjc.0000000000000157. Critical Role Of Prohibitin In Endothelial Cell Apoptosis Caused By Glycated Low-Den...
Zhou Y, Tan X, Kuang W, Liu L, Wan L. Transl Res. 2012 Jun;159(6):464-72. Doi: 10.1016/J.Trsl.2011.09.007. Epub 2011 Oct 24. Erythromycin Ameliorates Cigarette-Smoke-Induced Emphysema And Inflammation In Rats.
Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.
Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.
Wang Jm, Xiao Bl, Zheng Jw, Chen Hb, Zou Sq. World J Gastroenterol. 2007 Jun 21;13(23):3171-5. Effect Of Targeted Magnetic Nanoparticles Containing 5-Fu On Expression Of Bcl-2, Bax And Caspase 3 In Nude Mice With Transplanted Human Liver Cancer.
Meng Qt, Xia Zy, Luo T, Cao C, Zhao B, Wu Y, Wu X, Chen X. Saudi Med J. 2012 Feb;33(2):139-45. Ligustrazine Attenuates Acute Lung Injury Induced By Blunt Chest Trauma.
Zhou Y, Li W, Xu Q, Huang Y. J Exp Clin Cancer Res. 2010 Oct 4;29:131. Doi: 10.1186/1756-9966-29-131. Elevated Expression Of Dickkopf-1 Increases The Sensitivity Of Human Glioma Cell Line Shg44 To Bcnu.
Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...
Peng X, Chen K, Chen J, Fang J, Cui H, Zuo Z, Deng J, Chen Z, Geng Y, Lai W. Environ Toxicol. 2015 Mar 4. Doi: 10.1002/Tox.22120. [Epub Ahead Of Print] Aflatoxin B1 Affects Apoptosis And Expression Of Bax, Bcl-2, And Caspase-3 In Thymus And Bursa ...
Chen J, Li X, Qiu J, You H, Zhang Q, Dong G, Zuo Y. Cell Biochem Biophys. 2013;67(3):1015-9. Doi: 10.1007/S12013-013-9597-5. Kinetics Of Apoptosis And Expression Of Apoptosis-Related Proteins In Rat Ca3 Hippocampus Cells After Experimental Diffuse...
Liu Y, Zhou Y, Wang Y, Geng D, Liu J. Obes Surg. 2011 Sep;21(9):1424-31. Doi: 10.1007/S11695-011-0388-Z. Roux-En-Y Gastric Bypass-Induced Improvement Of Glucose Tolerance And Insulin Resistance In Type 2 Diabetic Rats Are Mediated By Glucagon-Like...
Yang Y, Li Zh, Liu H, Shi Wd, Zhang J. Chin J Integr Med. 2015 Jun;21(6):423-30. Doi: 10.1007/S11655-014-1752-3. Epub 2014 May 10. Inhibitory Effect Of Tetramethylpyrazine Preconditioning On Overload Training-Induced Myocardial Apoptosis In Rats.
Song Gh, Wang Rl, Chen Zy, Zhang B, Wang Hl, Liu Ml, Gao Jp, Yan Xy. J Physiol Biochem. 2014 Sep;70(3):761-8. Doi: 10.1007/S13105-014-0344-1. Epub 2014 Jul 30. Toxic Effects Of Sodium Fluoride On Cell Proliferation And Apoptosis Of Leydig Cells Fr...
Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, Geng Y, Lai W. Food Chem Toxicol. 2013 Sep;59:446-54. Doi: 10.1016/J.Fct.2013.06.032. Epub 2013 Jun 28. Protective Role Of Sodium Selenite On Histopathological Lesions, D...
Mao Z, Song Z, Li G, Lv W, Zhao X, Li B, Feng X, Chen Y. Neural Regen Res. 2013 Jan 15;8(2):133-42. Doi: 10.3969/J.Issn.1673-5374.2013.02.005. 8-Hydroxy-2-(Di-N-Propylamino)Tetralin Intervenes With Neural Cell Apoptosis Following Diffuse Axonal In...
Yan Y, Liang B, Zhang J, Liu Y, Bu X. Mol Med Rep. 2015 Jan;11(1):317-26. Doi: 10.3892/Mmr.2014.2657. Epub 2014 Oct 15. Apoptotic Induction Of Lung Adenocarcinoma A549 Cells Infected By Recombinant Rvg Newcastle Disease Virus (Rl-Rvg) In Vitro.
Li X, Wang L, Wang Z. Phys Med. 2015 Jun;31(4):352-9. Doi: 10.1016/J.Ejmp.2015.02.004. Epub 2015 Feb 19. Radioprotective Activity Of Neutral Polysaccharides Isolated From The Fruiting Bodies Of Hohenbuehelia Serotina.
Wei T, Tian W, Yan H, Shao G, Xie G. Cell Mol Neurobiol. 2014 Nov;34(8):1165-73. Doi: 10.1007/S10571-014-0091-4. Epub 2014 Aug 22. Protective Effects Of Phillyrin On H2O 2-Induced Oxidative Stress And Apoptosis In Pc12 Cells.
Ding H, Wang H, Zhao Y, Sun D, Zhai X. Cell Mol Neurobiol. 2015 Jul;35(5):623-32. Doi: 10.1007/S10571-015-0156-Z. Epub 2015 Jan 18. Protective Effects Of Baicalin On A??????????????-Induced Learning And Memory Deficit, Oxidative Stress, And Apoptosis In Rat.
Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...
Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...
Duo J, Ying Gg, Wang Gw, Zhang L. Mol Med Rep. 2012 Jun;5(6):1453-6. Doi: 10.3892/Mmr.2012.845. Epub 2012 Mar 22. Quercetin Inhibits Human Breast Cancer Cell Proliferation And Induces Apoptosis Via Bcl-2 And Bax Regulation.
Li Ly, Zhang Lj, Jia Jy, Zhao Q, Wang Eb, Li Qw. Int J Clin Exp Pathol. 2013;6(2):212-23. Epub 2013 Jan 15. Does Dynamic Immobilization Reduce Chondrocyte Apoptosis And Disturbance To The Femoral Head Perfusion?
Ji Cf, Ji Yb. Oncol Lett. 2014 May;7(5):1728-1732. Epub 2014 Mar 7. Laminarin-Induced Apoptosis In Human Colon Cancer Lovo Cells.
Tang C, Lu Yh, Xie Jh, Wang F, Zou Jn, Yang Js, Xing Yy, Xi T. Anticancer Drugs. 2009 Apr;20(4):249-58. Doi: 10.1097/Cad.0B013E328327D476. Downregulation Of Survivin And Activation Of Caspase-3 Through The Pi3K/Akt Pathway In Ursolic Acid-Induced ...
Meng Fj, Jiao Sm, Yu B. Int J Mol Med. 2012 Aug;30(2):263-70. Doi: 10.3892/Ijmm.2012.987. Epub 2012 May 9. Picroside Ii Protects Cardiomyocytes From Hypoxia/Reoxygenation-Induced Apoptosis By Activating The Pi3K/Akt And Creb Pathways.
Tian W, Wei T, Li B, Wang Z, Zhang N, Xie G. Plos One. 2014 May 6;9(5):E96775. Doi: 10.1371/Journal.Pone.0096775. Ecollection 2014. Pathway Of Programmed Cell Death And Oxidative Stress Induced By ??-Hydroxybutyrate In Dairy Cow Abomasum Smooth Mus...
Zhang D, Fu M, Song C, Wang C, Lin X, Liu Y. Neural Regen Res. 2012 Oct 25;7(30):2347-53. Doi: 10.3969/J.Issn.1673-5374.2012.30.004. Expressions Of Apoptosis-Related Proteins In Rats With Focal Cerebral Ischemia After Angong Niuhuang Sticker Point...
Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...
Dong Hy, Wang Zf. Chin J Cancer Res. 2014 Apr;26(2):200-7. Doi: 10.3978/J.Issn.1000-9604.2014.04.07. Antitumor Effects Of Artesunate On Human Breast Carcinoma Mcf-7 Cells And Igf-Ir Expression In Nude Mice Xenografts.
Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induced...
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.