Anti-Bax Rabbit Monoclonal Antibody

BAX antibody

Boster Bio Anti-Bax Rabbit Monoclonal Antibody catalog # M00183-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00183-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IHC, WB

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Product Name

Anti-Bax Rabbit Monoclonal Antibody

View all BAX Antibodies

SKU/Catalog Number

M00183-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Bax Rabbit Monoclonal Antibody catalog # M00183-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bax Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00183-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAB-2

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Bax

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00183-1 is reactive to BAX in Human, Mouse, Rat

Applications

M00183-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

21.184kDa

Background of BAX

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For BAX (Source: Uniprot.org, NCBI)

Gene Name

BAX

Full Name

Apoptosis regulator BAX

Weight

21.184kDa

Superfamily

Bcl-2 family

Alternative Names

apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4 BAX BCL2L4 BCL2 associated X, apoptosis regulator apoptosis regulator BAX|BCL2 associated X protein|BCL2-associated X protein omega|Baxdelta2G9|Baxdelta2G9omega|Baxdelta2omega|bcl-2-like protein 4|bcl2-L-4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BAX, check out the BAX Infographic

BAX infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BAX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00183-1 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Meng K,Yuan G,Bao H,Wang L,Ma R,Yu B,Zhao S.Interaction of HCCR-1 and Bax in breast cancer.J BUON.2019 May-Jun;24(3):1027-1037.PMID:31424657.
Species: Human
M00183-1 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION: NA

Su-Su Tang,Yi Ren,Xiao-Qian Ren,Jing-Ran Cao,Hao Hong,Hui Ji,Qing-Hua Hu,ERα and/or ERβ activation ameliorates cognitive impairment, neurogenesis and apoptosis in type 2 diabetes mellitus mice,Experimental Neurology,Volume 311,2019,Pages 33-43,ISSN 0014-4886,https://doi.org/10.1016/j.expneurol.2018.09.002.
Species: Mouse
M00183-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:1:500

Ren Q,Hu Z,Jiang Y,Tan X,Botchway BOA,Amin N,Lin G,Geng Y,Fang M.SIRT1 Protects Against Apoptosis by Promoting Autophagy in the Oxygen Glucose Deprivation/Reperfusion-Induced Injury.Front Neurol.2019 Dec 5;10:1289.doi:10.3389/fneur.2019.01289.PMID:31920915;PMCID:PMC6915092.
Species: Mouse
M00183-1 usage in article: APP:WB, SAMPLE:PC12 CELL, DILUTION:1:400

Liang,T.,Wei,C.,Lu,S.,Qin,M.,Qin,G.,Zhang,Y. ... Yang,Y. (2021).Ginaton injection alleviates cisplatin‑induced renal interstitial fibrosis in rats via inhibition of apoptosis through regulation of the p38MAPK/TGF‑β1 and p38MAPK/HIF‑1α pathways.Biomedical
Species: Rat
M00183-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200

Li C,Liu W,Li X,Zhang Z,Qi H,Liu S,Yan N,Xing Y,Hölscher C,Wang Z.The novel GLP-1/GIP analogue DA5-CH reduces tau phosphorylation and normalizes theta rhythm in the icv. STZ rat model of AD.Brain Behav.2020 Mar;10(3):e01505.doi:10.1002/brb3.1505.Epub 2020
Species: Mouse,Rat
M00183-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:1:2500

Zhu N,Ruan J,Yang X,Huang Y,Jiang Y,Wang Y,Cai D,Geng Y,Fang M.Triptolide improves spinal cord injury by promoting autophagy and inhibiting apoptosis.Cell Biol Int.2020 Mar;44(3):785-794.doi:10.1002/cbin.11273.Epub 2019 Dec 9.PMID:31774600.
Species: Mouse
M00183-1 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:400

Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
Species: Human
M00183-1 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300

Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
M00183-1 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000

Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
M00183-1 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:1000

Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
M00183-1 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA

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16 Customer Q&As for Anti-Bax Rabbit Monoclonal Antibody

Question

We are currently using anti-Bax Rabbit Monoclonal antibody M00183-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

The anti-Bax Rabbit Monoclonal antibody (M00183-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-07

Question

Do you have a BSA free version of anti-Bax Rabbit Monoclonal antibody M00183-1 available?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bax Rabbit Monoclonal antibody M00183-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-01

Question

Please see the WB image, lot number and protocol we used for mucosa of transverse colon using anti-Bax Rabbit Monoclonal antibody M00183-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-21

Question

Will anti-Bax Rabbit Monoclonal antibody M00183-1 work for IP with mucosa of transverse colon?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

According to the expression profile of mucosa of transverse colon, BAX is highly expressed in mucosa of transverse colon. So, it is likely that anti-Bax Rabbit Monoclonal antibody M00183-1 will work for IP with mucosa of transverse colon.

Boster Scientific Support

Answered: 2020-02-14

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of transverse colon using anti-Bax Rabbit Monoclonal antibody M00183-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-05

Question

Our lab used your anti-Bax Rabbit Monoclonal antibody for IHC on mucosa of transverse colon a few months ago. I am using mouse, and We intend to use the antibody for IP next. I was wanting to use examining mucosa of transverse colon as well as b-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

I took a look at the website and datasheets of our anti-Bax Rabbit Monoclonal antibody and I see that M00183-1 has been tested on mouse in both IHC and IP. Thus M00183-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-06

Question

I was wanting to use your anti-Bax Rabbit Monoclonal antibody for IP for human mucosa of transverse colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mucosa of transverse colon identification?

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

As indicated on the product datasheet, M00183-1 anti-Bax Rabbit Monoclonal antibody has been validated for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human mucosa of transverse colon in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-01

Question

Is this M00183-1 anti-Bax Rabbit Monoclonal antibody reactive to the isotypes of BAX?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

The immunogen of M00183-1 anti-Bax Rabbit Monoclonal antibody is A synthesized peptide derived from human Bax. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-26

Question

Would M00183-1 anti-Bax Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-19

Answer

As indicated on the product datasheet, M00183-1 anti-Bax Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-19

Question

My question regarding product M00183-1, anti-Bax Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Krishna

Verified customer

Asked: 2018-08-27

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00183-1 anti-Bax Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-08-27

Question

I see that the anti-Bax Rabbit Monoclonal antibody M00183-1 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-05-24

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-24

Question

I am interested in using your anti-Bax Rabbit Monoclonal antibody for b cell negative selection studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-08-21

Answer

We appreciate your inquiry. This M00183-1 anti-Bax Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-08-21

Question

We need to test anti-Bax Rabbit Monoclonal antibody M00183-1 on human mucosa of transverse colon for research purposes, then I may be interested in using anti-Bax Rabbit Monoclonal antibody M00183-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-07-12

Answer

The products we sell, including anti-Bax Rabbit Monoclonal antibody M00183-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-07-12

Question

We have seen staining in mouse mucosa of transverse colon. Are there any suggestions? Is anti-Bax Rabbit Monoclonal antibody supposed to stain mucosa of transverse colon positively?

K. Krishna

Verified customer

Asked: 2016-12-21

Answer

From literature mucosa of transverse colon does express BAX. From Uniprot.org, BAX is expressed in mucosa of transverse colon, b-cell, brain, ovarian carcinoma, skin, among other tissues. Regarding which tissues have BAX expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8358790
Brain, Pubmed ID: 9920818
Ovarian carcinoma, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-12-21

Question

My colleagues were well pleased with the WB result of your anti-Bax Rabbit Monoclonal antibody. However we have seen positive staining in b-cell isoform beta: cytoplasm. using this antibody. Is that expected? Could you tell me where is BAX supposed to be expressed?

C. Mitchell

Verified customer

Asked: 2015-05-14

Answer

From what I have seen in literature, b-cell does express BAX. Generally BAX expresses in isoform alpha: mitochondrion outer membrane, isoform beta: cytoplasm., isoform gamma: cytoplasm., isoform delta: cytoplasm. Regarding which tissues have BAX expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8358790
Brain, Pubmed ID: 9920818
Ovarian carcinoma, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2015-05-14

Question

Is a blocking peptide available for product anti-Bax Rabbit Monoclonal antibody (M00183-1)?

H. Brown

Verified customer

Asked: 2014-03-19

Answer

We do provide the blocking peptide for product anti-Bax Rabbit Monoclonal antibody (M00183-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-03-19

Order DetailsPrice
M00183-1

100μL

$370
M00183-1-30ul

30μL sample

$99
M00183-1-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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