Data & Images
|Product Name||Anti-BCAT2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-BCAT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1502)|
|Replacement Item||This antibody may replace the following items: sc-134964|sc-169986 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT2(377-392aa KEIQYGIRAHEWMFPV).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT(m))|
|Molecular Weight||44288 MW|
|Protein Function||Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.|
|Sequence Similarities||Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.|
|Subcellular Localization||Isoform A: Mitochondrion.|
|Alternative Names||Branched-chain-amino-acid aminotransferase, mitochondrial;BCAT(m);220.127.116.11;Placental protein 18;PP18;BCAT2;BCATM, BCT2, ECA40;|
|Research Areas|||signal transduction|metabolism|amino acids| signal transduction|mitochondrial| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|metabolic signaling pathways|amino acid metabolism|types of disease|cancer||
Background for Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT(m))
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-BCAT2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-BCAT2 Antibody Images
Click the images to enlarge.
All lanes: Anti BCAT2 (PA1502) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
IHC(P): Human Colon Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,