|Sample Size:||30ug for $99, contact us for details|
|Application:||Flow Cytometry, IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-BCL2L2/Bcl W Antibody|
|Description||Rabbit IgG polyclonal antibody for Bcl-2-like protein 2(BCL2L2) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.|
|Cite This Product||Anti-BCL2L2/Bcl W Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1426-1)|
|Replacement Item||This antibody may replace the following items: sc-11422|sc-130701|sc-6172|sc-6418 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||Flow Cytometry, IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human BCL2L2(74-88aa SAQQRFTQVSDELFQ), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Bcl-2-like protein 2(Bcl2-L-2)|
|Molecular Weight||20746 MW|
|Protein Function||Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. .|
|Tissue Specificity||Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. .|
|Sequence Similarities||Belongs to the Bcl-2 family.|
|Subcellular Localization||Mitochondrion membrane ; Peripheral membrane protein . Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.|
|Alternative Names||Bcl-2-like protein 2;Bcl2-L-2;Apoptosis regulator Bcl-W;BCL2L2;BCLW, KIAA0271;|
|Research Areas|||cell biology|apoptosis|intracellular|bcl2 family| cancer|oncoproteins/suppressors|oncoproteins|cell survival & death||
Background for Bcl-2-like protein 2(Bcl2-L-2)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-BCL2L2/Bcl W Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-BCL2L2/Bcl W Antibody Images
Click the images to enlarge.
All lanes: Anti BCL2L2 (PA1426-1) at 0.5ug/ml
WB: HELAWhole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
IHC(P): Human Rectal Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,