|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Bcl3 Antibody|
|Description||Rabbit IgG polyclonal antibody for B-cell lymphoma 3 protein(BCL3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Bcl3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1539)|
|Replacement Item||This antibody may replace the following items: sc-20986|sc-25294|sc-21417 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Bcl3(199-216aa MALDRHGQTAAHLACEHR), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||B-cell lymphoma 3 protein(BCL-3)|
|Molecular Weight||47584 MW|
|Protein Function||Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity). .|
|Sequence Similarities||Contains 7 ANK repeats.|
|Subcellular Localization||Nucleus. Cytoplasm . Cytoplasm, perinuclear region . Ubiquitination via 'Lys- 63'-linked ubiquitin chains is required for nuclear accumulation. .|
|Alternative Names||B-cell lymphoma 3 protein;BCL-3;Proto-oncogene BCL3;BCL3;BCL4, D19S37;|
|Research Areas|||immunology|adaptive immunity|b cells|non-cd| epigenetics and nuclear signaling|transcription|cancer susceptibility|proto-oncogenes|nuclear signaling pathways|nfkb pathway||
Background for B-cell lymphoma 3 protein(BCL-3)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Bcl3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Bcl3 Antibody Images
Click the images to enlarge.
Lane 1: JURKAT Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: CEM Cell Lysate
IHC(P): Human Lung Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,