Rabbit Polyclonal antibody for TNFRSF17 detection. Tested positive for ELISA, IF, IHC, WB in Human
|Applications||ELISA, IF, IHC, WB|
|Product Name||Anti-BCMA Antibody
See all TNFRSF17 primary antibodies, ELISA kits and proteins
|Storage & Handling||-20°C|
|Description||Rabbit Polyclonal antibody for TNFRSF17 detection. Tested positive for ELISA, IF, IHC, WB in Human|
|Cite This Product||Anti-BCMA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01014)|
|Specificity||Anti-BCMA Antibody (A01014) reacts with Human TNFRSF17, in native form and recombinant. Superfamily members of TNFRSF17 are not reactive to A01014.|
|Contents/Buffer||Liquid. In PBS containing 0.02% sodium azide.|
|Form||Liquid. In PBS containing 0.02% sodium azide.|
|Immunogen||Synthetic peptide corresponding to the sequence near the C-terminus of human BCMA.|
Our Boster Quality Guarantee for Anti-BCMA Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry (paraffin sections, 10µg/ml)
Western Blot (2-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Images And Assay Conditions
Immunofluorescence of BCMA in human spleen cells with BCMA antibody at 10
Figure 3. IHC analysis of TNFRSF17 using anti-TNFRSF17 antibody (A01014).
TNFRSF17 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNFRSF17 Antibody (A01014) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. Western blot analysis of TNFRSF17 using anti-TNFRSF17 antibody (A01014).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF17 antigen affinity purified polyclonal antibody (Catalog # A01014) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for TNFRSF17.
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
*You can search these to find other products in these research areas.
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