Data & Images
|Product Name||Anti-BCRP/ABCG2 Antibody|
|Description||Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family G member 2(ABCG2) detection. Tested with WB in Human.|
|Cite This Product||Anti-BCRP/ABCG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1869)|
|Replacement Item||This antibody may replace the following items: sc-25821|sc-58224|sc-58222|sc-130933|sc-377176|sc-69988|sc-69989|sc-18841|sc-25822|sc-25156 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human BCRP/ABCG2(154-168aa NHEKNERINRVIQEL).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||ATP-binding cassette sub-family G member 2|
|Molecular Weight||72314 MW|
|Protein Function||High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. Implicated in the efflux of numerous drugs and xenobiotics: mitoxantrone, the photosensitizer pheophorbide, camptothecin, methotrexate, azidothymidine (AZT), and the anthracyclines daunorubicin and doxorubicin. .|
|Tissue Specificity||Highly expressed in placenta. Low expression in small intestine, liver and colon. .|
|Sequence Similarities||Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.|
|Alternative Names||ATP-binding cassette sub-family G member 2;Breast cancer resistance protein;CDw338;Mitoxantrone resistance-associated protein;Placenta-specific ATP-binding cassette transporter;Urate exporter;CD338;ABCG2;ABCP, BCRP, BCRP1, MXR;|
|Research Areas|||tags & cell markers|cell type markers|tumor associated| signal transduction|metabolism|plasma membrane|atpases| stem cells|neural stem cells|surface molecules|hematopoietic progenitors|drug metabolism| cancer|drug resistance|mrp-related proteins| metabolism|pathways and processes|metabolic signaling pathways|types of disease||
Background for ATP-binding cassette sub-family G member 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-BCRP/ABCG2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-BCRP/ABCG2 Antibody Images
Click the images to enlarge.
All lanes: Anti BCRP/ABCG2 (PA1869) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 72KD
Observed bind size: 72KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,