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SKU:PB9075
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-BDNF Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Brain-derived neurotrophic factor(BDNF) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-BDNF Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9075)
Replacement Item This antibody may replace the following items: sc-546|sc-33905|sc-33904|sc-20981 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Bovine, Canine, Horse, Monkey, Rabbit

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Bdnf
Protein Name Brain-derived neurotrophic factor
Molecular Weight 28123 MW
Protein Function During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.
Subcellular Localization Secreted.
Uniprot ID P21237
Alternative Names Brain-derived neurotrophic factor;BDNF;Bdnf;
Research Areas |neuroscience|neurology process|growth and development|neurotrophins| neuroscience|neurodegenerative disease|huntington's disease|alzheimer's disease| cardiovascular|atherosclerosis|diabetes associated|thrombosis| metabolism|types of disease|diabetes|obesity|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Brain-derived neurotrophic factor

Brain-derived neurotrophic factor, also known as BDNF, is a secreted protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. It is mapped to 11p14.1. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

Anti-BDNF Picoband™ Antibody Images

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Anti-BDNF Picoband™ Antibody
Anti-BDNF Picoband antibody, PB9075-1.JPG
IHC(P): Rat Brain Tissue
Anti-BDNF Picoband™ Antibody
Anti-BDNF Picoband antibody, PB9075-2.JPG
IHC(P): Mouse Brain Tissue
Anti-BDNF Picoband™ Antibody
Anti-BDNF Picoband antibody, PB9075-3.jpg
All lanes: Anti-BDNF(PB9075) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD

Customer Reviews

Review by Nancy Tseng
Quality

Source: Biocompare.com

"Shows good bands on the positive control"

--Nancy Tseng, NEUROSURGERY, UC-DENVER, SENIOR-PRA

Applications Western Blot
Sample Hek293
Detection ECL

"This anti-BDNF antibody (Boster-PB9075) works great on the positive control every time, but we still have not received results for our samples. Needs troubleshooting...Still waiting on results for our samples."

(Posted on 8/23/2016)
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to bdnf antibody, brain derived neurotrophic factor antibody, brain-derived neurotrophic factor antibody