Data & Images
|Product Name||Anti-BDNF Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Brain-derived neurotrophic factor(BDNF) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-BDNF Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9075)|
|Replacement Item||This antibody may replace the following items: sc-546|sc-33905|sc-33904|sc-20981 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Predicted Species||Bovine, Canine, Horse, Monkey, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Brain-derived neurotrophic factor|
|Molecular Weight||28123 MW|
|Protein Function||During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.|
|Alternative Names||Brain-derived neurotrophic factor;BDNF;Bdnf;|
|Research Areas|||neuroscience|neurology process|growth and development|neurotrophins| neuroscience|neurodegenerative disease|huntington's disease|alzheimer's disease| cardiovascular|atherosclerosis|diabetes associated|thrombosis| metabolism|types of disease|diabetes|obesity||
Background for Brain-derived neurotrophic factor
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-BDNF Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-BDNF Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Rat Brain Tissue
IHC(P): Mouse Brain Tissue
All lanes: Anti-BDNF(PB9075) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
- Review by Nancy Tseng
"Shows good bands on the positive control"
--Nancy Tseng, NEUROSURGERY, UC-DENVER, SENIOR-PRA
Applications Western Blot Sample Hek293 Detection ECL
"This anti-BDNF antibody (Boster-PB9075) works great on the positive control every time, but we still have not received results for our samples. Needs troubleshooting...Still waiting on results for our samples."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,