Anti-BDNF Rabbit Monoclonal Antibody

Bdnf antibody

Boster Bio Anti-BDNF Rabbit Monoclonal Antibody catalog # M00035-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00035-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-BDNF Rabbit Monoclonal Antibody

View all Bdnf Antibodies

SKU/Catalog Number

M00035-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-BDNF Rabbit Monoclonal Antibody catalog # M00035-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BDNF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00035-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BEH-2

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human BDNF

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00035-1 is reactive to BDNF in Human, Mouse, Rat

Applications

M00035-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

27.818kDa

Background of Bdnf

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For BDNF (Source: Uniprot.org, NCBI)

Gene Name

BDNF

Full Name

Brain-derived neurotrophic factor

Weight

27.818kDa

Superfamily

NGF-beta family

Alternative Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BDNF, check out the BDNF Infographic

BDNF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BDNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00035-1 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xue LL,Du RL,Hu Y,Xiong LL,Su ZY,Ma Z,Tan YX,Liu J,Hu Q,Zhu ZQ,Wang TH,Liu XZ.BDNF promotes neuronal survival after neonatal hypoxic-ischemic encephalopathy by up-regulating Stx1b and suppressing VDAC1.Brain Res Bull.2021 May 28:S0361-9230(21)00144-1.doi:10.1016/j.brainresbull.2021.05.013.Epub ahead of print.PMID:34058286.
Species: Rat
M00035-1 usage in article: APP:WB, SAMPLE:CORTEX AND HIPPOCAMPUS, DILUTION:1:100

Huangqin flavonoid extraction for spinal cord injury in a rat model

Serotonin regulates brain-derived neurotrophic factor expression in select brain regions during acute psychological stress

Beneficial effect of fluoxetine treatment aganist psychological stress is mediated by increasing BDNF expression in selected brain areas

Brain-derived neurotrophic factor is up-regulated in severe acute cauda equina syndrome dog model

Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles

Acute Stress and Chronic Stress Change Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Kinase-Coupled Receptor (TrkB) Expression in Both Young and Aged Rat Hippocampus

Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion

Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory

Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury

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Order DetailsPrice
M00035-1

100μL

$370
M00035-1-30ul

30μL sample

$99
M00035-1-carrier-free

Carrier Free

$370

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