Product Info Summary
SKU: | M01263-Biotin |
---|---|
Size: | 0.1 mg |
Reactive Species: | Chicken, Drosophila, Human, Mouse, Rabbit, Rat, Zebrafish |
Host: | Mouse |
Application: | ELISA, WB |
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Product info
Product Name
Anti-beta-Actin ACTB Monoclonal Antibody [10B7] (biotin)
View all beta-Actin Antibodies
SKU/Catalog Number
M01263-Biotin
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-beta-Actin ACTB Monoclonal Antibody [10B7] (biotin) (Catalog # M01263-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken, Zebrafish, Drosophila.
Storage & Handling
beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-beta-Actin ACTB Monoclonal Antibody [10B7] (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-Biotin)
Host
Mouse
Contents
beta-Actin antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 10B7
Isotype
IgG
Immunogen
beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of beta-actin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Reactive Species
M01263-Biotin is reactive to ACTB in Chicken, Drosophila, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
M01263-Biotin is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.737kDa
Background of beta-Actin
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
beta-Actin antibody can be used for detection of Beta-Actin by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Beta-Actin in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, THP-1, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, rat lung, rat liver, rabbit liver, rabbit spleen, chicken liver, chicken small intestine, zebrafish, and drosophila lysate with beta-Actin antibody at 1 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41.737kDa
Superfamily
actin family
Alternative Names
ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta-Actin ACTB Monoclonal Antibody [10B7] (biotin) (M01263-Biotin)
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No publications found for M01263-Biotin
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-beta-Actin ACTB Monoclonal Antibody [10B7] (biotin)
Question
Can M01263-Biotin be used for the IHC-P technique?
Verified customer
Asked: 2022-03-07
Answer
Anti-Beta-Actin ACTB Monoclonal Antibody [10B7] (Biotin) (M01263-Biotin) has not been tested on IHC-P. We cannot extend a guarantee for IHC-P for it. However if the customer decides to try and it works for them and they are willing to share the results with us, we can issue the product credit to them as per our innovating scientist award. Overall the recommendation is against using this antibody on IHC-P but we would assist in ways we can if the customer wants to try it.
Boster Scientific Support
Answered: 2022-03-07