Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody

beta-III Tubulin antibody

Boster Bio Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody catalog # M01857-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M01857-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody

View all beta-III Tubulin Antibodies

SKU/Catalog Number

M01857-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody catalog # M01857-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01857-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CC-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human beta III Tubulin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01857-1 is reactive to TUBB3 in Human, Mouse, Rat

Applications

M01857-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

50.433kDa

Background of beta-III Tubulin

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For TUBB3 (Source: Uniprot.org, NCBI)

Gene Name

TUBB3

Full Name

Tubulin beta-3 chain

Weight

50.433kDa

Superfamily

tubulin family

Alternative Names

Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen TUBB3 CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4 tubulin beta 3 class III tubulin beta-3 chain|class III beta-tubulin|tubulin beta-4 chain|tubulin beta-III|tubulin, beta 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TUBB3, check out the TUBB3 Infographic

TUBB3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TUBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01857-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Karami S,Doroodmand MM,Taherianfar M,Mutabi-Alavi A,Nagshgar N.Mechanism behind the neuronal ephaptic coupling during synchronizing by specific brain-triggered wave as neuronal motor toolkit.Sci Rep.2021 Feb 11;11(1):3683.doi:10.1038/s41598-021-82118-2.PM
Species: Rat,Snail

Karami S,Doroodmand MM,Taherianfar M,Mutabi-Alavi A,Nagshgar N.Mechanism behind the neuronal ephaptic coupling during synchronizing by specific brain-triggered wave as neuronal motor toolkit.Sci Rep.2021 Feb 11;11(1):3683.doi:10.1038/s41598-021-82118-2.PM
Species: Rat,Snail

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4 Customer Q&As for Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody

Question

We are currently using anti-beta III Tubulin Rabbit Monoclonal antibody M01857-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

The anti-beta III Tubulin Rabbit Monoclonal antibody (M01857-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-06

Question

I was wanting to use your anti-beta III Tubulin Rabbit Monoclonal antibody for WB for mouse hypothalamus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse hypothalamus identification?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

As indicated on the product datasheet, M01857-1 anti-beta III Tubulin Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse hypothalamus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-28

Question

Here is the WB image, lot number and protocol we used for hypothalamus using anti-beta III Tubulin Rabbit Monoclonal antibody M01857-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-21

Question

Does anti-beta III Tubulin Rabbit Monoclonal antibody M01857-1 work for WB with hypothalamus?

Verified Customer

Verified customer

Asked: 2017-08-23

Answer

According to the expression profile of hypothalamus, TUBB3 is highly expressed in hypothalamus. So, it is likely that anti-beta III Tubulin Rabbit Monoclonal antibody M01857-1 will work for WB with hypothalamus.

Boster Scientific Support

Answered: 2017-08-23

Order DetailsPrice
M01857-1

100μL

$370
M01857-1-30ul

30μL sample

$99
M01857-1-carrier-free

Carrier Free

$370

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M01857-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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