Anti-Beta TrCP2 FBXW11 Antibody

FBXW11 antibody

Boster Bio Anti-Beta TrCP2 FBXW11 Antibody (Catalog # A05523). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A05523
Size: 100ug
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Beta TrCP2 FBXW11 Antibody

View all FBXW11 Antibodies

SKU/Catalog Number

A05523

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Beta TrCP2 FBXW11 Antibody (Catalog # A05523). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Beta TrCP2 FBXW11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05523)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human ßTrCP2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05523 is reactive to FBXW11 in Human, Mouse

Applications

A05523 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

62.091kDa

Background of FBXW11

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 – 1:100,000
IF Microscopy: User optimized
WB: 1:200 to 1:1,000

Validation Images & Assay Conditions

Gene/Protein Information For FBXW11 (Source: Uniprot.org, NCBI)

Gene Name

FBXW11

Full Name

F-box/WD repeat-containing protein 11

Weight

62.091kDa

Alternative Names

beta-transducin repeat-containing protein 2; BTRC2F-box/WD repeat-containing protein 1B; BTRCP2FBW1B; F-box and WD repeat domain containing 11; F-box and WD-40 domain protein 11; F-box and WD-40 domain protein 1B; F-box protein Fbw1b; F-box/WD repeat-containing protein 11; Fbw11; FBXW1BFbw1b; Homologous to Slimb protein; Hos; KIAA0696F-box and WD repeats protein beta-TrCP2 FBXW11 BTRC2, BTRCP2, FBW1B, FBXW1B, Fbw11, Hos, NEDJED F-box and WD repeat domain containing 11 F-box/WD repeat-containing protein 11|F-box and WD repeats protein beta-TrCP2|F-box and WD-40 domain protein 11|F-box and WD-40 domain protein 1B|F-box protein Fbw1b|F-box/WD repeat-containing protein 1B|beta-transducin repeat-containing protein 2|homologous to Slimb protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FBXW11, check out the FBXW11 Infographic

FBXW11 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FBXW11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Beta TrCP2 FBXW11 Antibody

Question

Can you help my question with product A05523, anti-Beta TrCP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05523 anti-Beta TrCP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-09

Question

We are currently using anti-Beta TrCP2 antibody A05523 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

The anti-Beta TrCP2 antibody (A05523) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-31

Question

Is a blocking peptide available for product anti-Beta TrCP2 antibody (A05523)?

Verified Customer

Verified customer

Asked: 2018-02-16

Answer

We do provide the blocking peptide for product anti-Beta TrCP2 antibody (A05523). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-02-16

Order DetailsPrice
A05523

100 μg

$599

Free Secondary Antibody

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

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