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SKU:MA1112
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:TUB 2.1
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-Beta-Tubulin Antibody (Monoclonal, TUB 2.1)
SKU/Catalog Number MA1112
Description Mouse IgG monoclonal antibody for beta-Tubulin detection. Tested with WB, IHC-P in Human,mouse,rat. No cross reactivity with other proteins.
Cite This Product Anti-Beta-Tubulin Antibody (Monoclonal, TUB 2.1) TUB 2.1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1112)
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Mouse, Rat
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Tubulin from rat brain.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number TUB 2.1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TUBB1
Protein Name Tubulin beta-1 chain
Molecular Weight 50327 MW
Protein Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). .
Tissue Specificity Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. .
Subcellular Localization Cytoplasm, cytoskeleton .
Uniprot ID Q9H4B7
Alternative Names Tubulin beta-1 chain;TUBB1;
Research Areas |tags & cell markers|subcellular markers|cytoskeleton|microtubules| signal transduction|cytoskeleton / ecm|tubulin| isotype/loading controls|beta tubulin|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tubulin beta-1 chain

Tubulin beta(TUBB) was derived from its corresponding mRNA with a molecular weight of about 55,000. TUBB gene contains 4 exons and located in the HLA class I region at 6p21.3. Paclitaxel resistance in non-small-cell lung cancer associated with beta-tubulin gene mutations.

Anti-Beta-Tubulin Antibody (Monoclonal, TUB 2.1) Images

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Anti-Beta-Tubulin Antibody (Monoclonal, TUB 2.1)
Anti-beta-Tubulin antibody (monoclonal), MA1112, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate
Anti-Beta-Tubulin Antibody (Monoclonal, TUB 2.1)
Anti-beta-Tubulin antibody (monoclonal), MA1112, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Wang, Y., Xu, C., Xu, Z., Ji, C., Liang, J., Wang, Y.,…, & Chen, Z. (2017). Depolarized GABAergic Signaling in Subicular Microcircuits Mediates Generalized Seizure in Temporal Lobe Epilepsy. Neuron, 95(1), 92-105.e5. doi: 10.1016/j.neuron.2017.06.004
Ye Y, Zeng Ym, Wan Mr, Lu Xf. Cell Biochem Biophys. 2011 Apr;59(3):179-84. Doi: 10.1007/S12013-010-9130-Z. Induction Of Human Bone Marrow Mesenchymal Stem Cells Differentiation Into Neural-Like Cells Using Cerebrospinal Fluid.
Chen Xh, Bao Yy, Zhou Sh, Wang Qy, Wei Y, Fan J. Mol Med Rep. 2013 Dec;8(6):1695-700. Doi: 10.3892/Mmr.2013.1740. Epub 2013 Oct 18. Glucose Transporter-1 Expression In Cd133+ Laryngeal Carcinoma Hep-2 Cells.
Zuo Q, Liu J, Zhang J, Wu M, Guo L, Liao W. Sci Rep. 2015 Jun 25;5:11634. Doi: 10.1038/Srep11634. Development Of Trastuzumab-Resistant Human Gastric Carcinoma Cell Lines And Mechanisms Of Drug Resistance.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tubb antibody, tubb2a antibody