|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated tested for WB in Human, Mouse, Rat|
|Cite This Product||Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H01857)|
|Contents/Buffer||Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Immunogen||A synthesized peptide derived from human beta Tubulin|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Images And Assay Conditions
Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate (H01857).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody (Catalog # H01857) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3
Protein Target Info (Source: Uniprot.org)
|Protein Name||Tubulin beta-3 chain|
|Tissue Specificity||Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. .|
|Alternative Names||Tubulin beta-3 chain;Tubulin beta-4 chain;Tubulin beta-III;TUBB3;TUBB4;|
|Subcellular Localization||Cytoplasm, cytoskeleton.|
|Molecular Weight||50433 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. .|
|Research Areas||Cell Adhesion Proteins, Cell Type Markers, Cytoskeleton, Cytoskeleton / Ecm, Microtubules, Neuroscience, Signal Transduction, Tubulin
*You can search these to find other products in these research areas.
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