SKU M05613
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number BI-20
Host Rabbit
Applications IF, IHC, WB

Overview

Product Name Anti-beta Tubulin Rabbit Monoclonal Antibody
SKU/Catalog Number M05613
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-beta Tubulin Rabbit Monoclonal Antibody tested for Flow Cytometry, IF, IHC, ICC, WB in Human, Mouse, Rat
Cite This Product Anti-beta Tubulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05613)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human beta Tubulin
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:3000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Flow Cytometry 1:100

Images And Assay Conditions

/M/0/M05613 TUBB primary antibodies IF testing 1.jpg

Immunofluorescent analysis of NIH/3T3 cells, using beta Tubulin Antibody

/M/0/M05613 TUBB primary antibodies IHC testing 1.jpg

Immunohistochemical analysis of paraffin-embedded (1) Human glioma; (2) Rat brain; (3) Mouse kidney; (4) Human uterus cancer, using beta Tubulin Antibody(M05613)
TUBB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB Antibody (M05613)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/M/0/M05613 TUBB primary antibodies WB testing 1.jpg

Western blot analysis of beta Tubulin expression in (1) MCF-7 cell lysate; (2) COS-1 cell lysate; (3) Jurkat cell lysate; (4) HeLa cell lysate (M05613).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB monoclonal antibody (Catalog # M05613) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P07437
Gene Name TUBB
Protein Name Tubulin beta chain
Tissue Specificity Ubiquitously expressed with highest levels in spleen, thymus and immature brain. .
Alternative Names Tubulin beta chain;Tubulin beta-5 chain;TUBB;TUBB5;OK/SW-cl.56;
Subcellular Localization Cytoplasm, cytoskeleton.
Molecular Weight 49671 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Research Areas Cytoskeleton, Cytoskeleton / Ecm, Microtubules, Signal Transduction, Subcellular Markers, Tags & Cell Markers, Tubulin

*You can search these to find other products in these research areas.

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Order Product (M05613)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

Ships in 7-10 business days.

Troubleshooting

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Publications

Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats
The Potential Role of HMGB1 Release in Peritoneal Dialysis-Related Peritonitis