Anti-Bid Antibody

BID antibody

Boster Bio Anti-Bid Antibody catalog # PA2015. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA2015
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, WB

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Product Name

Anti-Bid Antibody

View all BID Antibodies

SKU/Catalog Number

PA2015

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bid Antibody catalog # PA2015. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bid Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2015)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid, different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2015 is reactive to BID in Mouse, Rat

Applications

PA2015 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

21.995kDa

Background of BID

BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat By Heat
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For BID (Source: Uniprot.org, NCBI)

Gene Name

BID

Full Name

BH3-interacting domain death agonist

Weight

21.995kDa

Alternative Names

apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID BID FP497 BH3 interacting domain death agonist BH3-interacting domain death agonist|Human BID coding sequence|apoptic death agonist|desmocollin type 4|p22 BID

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BID, check out the BID Infographic

BID infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BID: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2015 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang Xb, Liu Qh, Mi N, Wang P, Tang W, Zhao Xh, Li Xj. Ultrasound Med Biol. 2010 Apr;36(4):667-76. Doi: 10.1016/J.Ultrasmedbio.2009.09.006. Epub 2010 Mar 7. Sonodynamically Induced Apoptosis By Protoporphyrin Ix On Hepatoma-22 Cells In Vitro.

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16 Customer Q&As for Anti-Bid Antibody

Question

We are currently using anti-Bid antibody PA2015 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

The anti-Bid antibody (PA2015) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-30

Question

Our lab want to know about to test anti-Bid antibody PA2015 on rat brain skin for research purposes, then I may be interested in using anti-Bid antibody PA2015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

The products we sell, including anti-Bid antibody PA2015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-05

Question

See attached the WB image, lot number and protocol we used for brain skin using anti-Bid antibody PA2015. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-15

Question

My boss were happy with the WB result of your anti-Bid antibody. However we have seen positive staining in blood isoform 2: mitochondrion membrane using this antibody. Is that expected? Could you tell me where is BID supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

From literature, blood does express BID. Generally BID expresses in cytoplasm., bh3-interacting domain death agonist p15:, bh3-interacting domain death agonist p13:, isoform 1: cytoplasm, isoform 3: cytoplasm, isoform 2: mitochondrion membrane. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-10-21

Question

I see that the anti-Bid antibody PA2015 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-08

Question

Is there a BSA free version of anti-Bid antibody PA2015 available?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bid antibody PA2015 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-25

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain skin using anti-Bid antibody PA2015. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-01

Question

We have been able to see staining in rat blood. Are there any suggestions? Is anti-Bid antibody supposed to stain blood positively?

W. Singh

Verified customer

Asked: 2019-01-08

Answer

According to literature blood does express BID. According to Uniprot.org, BID is expressed in blood, brain skin, embryonic kidney, liver, among other tissues. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-01-08

Question

Is a blocking peptide available for product anti-Bid antibody (PA2015)?

Verified Customer

Verified customer

Asked: 2018-11-12

Answer

We do provide the blocking peptide for product anti-Bid antibody (PA2015). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-12

Question

Would PA2015 anti-Bid antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-08-06

Answer

As indicated on the product datasheet, PA2015 anti-Bid antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-06

Question

Will anti-Bid antibody PA2015 work for IHC with brain skin?

Verified Customer

Verified customer

Asked: 2018-08-02

Answer

According to the expression profile of brain skin, BID is highly expressed in brain skin. So, it is likely that anti-Bid antibody PA2015 will work for IHC with brain skin.

Boster Scientific Support

Answered: 2018-08-02

Question

Is this PA2015 anti-Bid antibody reactive to the isotypes of BID?

Verified Customer

Verified customer

Asked: 2018-05-25

Answer

The immunogen of PA2015 anti-Bid antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid(179-195aa NQNLFSYVRNLVRNEMD), different from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-05-25

Question

I have a question about product PA2015, anti-Bid antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-13

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2015 anti-Bid antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-13

Question

We purchased anti-Bid antibody for WB on embryonic kidney a few years ago. I am using rat, and We intend to use the antibody for IHC next. I would like examining embryonic kidney as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

D. Thomas

Verified customer

Asked: 2017-07-27

Answer

I have checked the website and datasheets of our anti-Bid antibody and I see that PA2015 has been validated on rat in both WB and IHC. Thus PA2015 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-07-27

Question

My lab would like using your anti-Bid antibody for protein-containing complex assembly studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

We appreciate your inquiry. This PA2015 anti-Bid antibody is tested on rat spleen tissue, tissue lysate, mouse brain, brain tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-06-15

Question

I was wanting to use your anti-Bid antibody for IHC for rat brain skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain skin identification?

G. Brown

Verified customer

Asked: 2013-05-17

Answer

As indicated on the product datasheet, PA2015 anti-Bid antibody has been validated for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain skin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-05-17

Order DetailsPrice
PA2015

100μg

$370
PA2015-10ug

10μg sample (liquid)

$99
PA2015-Biotin

100 μg Biotin conjugated

$570
PA2015-Cy3

100 μg Cy3 conjugated

$570
PA2015-Dylight488

100 μg Dylight488 conjugated

$570
PA2015-Dylight550

100 μg Dylight550 conjugated

$570
PA2015-Dylight594

100 μg Dylight594 conjugated

$570
PA2015-FITC

100 μg FITC conjugated

$570
PA2015-HRP

100 μg HRP conjugated

$570
PA2015-APC

100 μg APC conjugated

$670
PA2015-PE

100 μg PE conjugated

$670
PA2015-carrier-free

Carrier Free

$370

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PA2015
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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