Overview
| Product Name | Anti-BIN1 Antibody (Monoclonal, 99D) |
|---|---|
| SKU/Catalog Number | MA1005 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins. |
| Cite This Product | Anti-BIN1 Antibody (Monoclonal, 99D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Form | Lyophilized |
| Immunogen | Recombinant polypeptide containing amino acids 189-398 of human Bin1. |
| Reactivity | Human, Mouse, Rat |
Assay Details
Assay Dilutions Overview
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry(Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.Images And Assay Conditions
Anti-BIN1 antibody (monoclonal), MA1005, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Figure 2. Western blot analysis of BIN1 using anti-BIN1 antibody (MA1005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysate,
Lane 2: mouse skeletal muscle tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BIN1 antigen affinity purified monoclonal antibody (Catalog # MA1005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BIN1 at approximately 65KD. The expected band size for BIN1 is at 65KD.
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | O08839 |
|---|---|
| Gene Name | BIN1 |
| Protein Name | Myc box-dependent-interacting protein 1 |
| Tissue Specificity | Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed. |
| Alternative Names | Myc box-dependent-interacting protein 1;Amphiphysin II;Amphiphysin-like protein;Bridging integrator 1;Bin1;Amph2, Amphl; |
| Subcellular Localization | Cytoplasm . Nucleus . |
| Molecular Weight | 64533 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. . |
|---|---|
| Research Areas | Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics And Nuclear Signaling, Host Virus Interaction, Interspecies Interaction, Microbiology, Oncoproteins/Suppressors, Transcription, Tumor Suppressors *You can search these to find other products in these research areas. |
| Background | BIN1(AMPH2) is a novel human gene product with features of a tumor suppressor protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei. |
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