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Breast Cancer Regulation
Mouse Monoclonal BIN1 Antibody (Monoclonal, 99D). Validated in IHC, ICC, WB and tested in Human, Mouse, Rat.
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Our Boster Quality Guarantee for Anti-BIN1 Antibody (Monoclonal, 99D) covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
The following reagents are used to generate the images below for Anti-BIN1 Antibody (Monoclonal, 99D) (MA1005).
Anti-BIN1 antibody (monoclonal), MA1005, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Skeletal Muscle Tissue LysateLane 3: Rat Heart Tissue LysateLane 4: Rat Kidney Tissue Lysate
Figure 2. Western blot analysis of BIN1 using anti-BIN1 antibody (MA1005). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysate, Lane 2: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BIN1 antigen affinity purified monoclonal antibody (Catalog # MA1005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BIN1 at approximately 65KD. The expected band size for BIN1 is at 65KD.
*if product is indicated to react with multiple species, protein info is based on the human gene.
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