Overview
| Product Name |
Anti-BIN1 Antibody (Monoclonal, 99D)
See more Bin1 products |
| Catalog# |
MA1005 |
| Pack Size |
100μg/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-BIN1 Antibody (Monoclonal, 99D) catalog # MA1005. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody. |
| Cite This Product |
Anti-BIN1 Antibody (Monoclonal, 99D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-BIN1 Antibody (Monoclonal, 99D) may replace the following items: sc 9360|sc 6320|sc 6319|sc 162325|sc 162326|sc 162327|sc 6319|sc 6320|sc 7015|sc 8357|sc 9360. |
Product Specs
| Host |
Mouse |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
Recombinant polypeptide containing amino acids 189-398 of human Bin1. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For Bin1 (Source: Uniprot.org, NCBI)
| Uniprot Id | O08839 |
|---|
| NCBI Gene Id | 117028 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | Bin1 |
|---|
| Protein Name | Myc box-dependent-interacting protein 1 |
|---|
| Alternative Names | Bin1|AMPH2; Amphiphysin II; Amphiphysin-like protein; AMPHLDKFZp547F068; box dependant MYC interacting protein 1; bridging integrator 1Box-dependent myc-interacting protein 1; MGC10367; myc box-dependent-interacting protein 1; SH3P9 |
|---|
| Molecular Weight | 64533 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Bin1, check out the Bin1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Bin1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Bin1 infographic
Our Boster Quality Guarantee for Anti-BIN1 Antibody (Monoclonal, 99D) covers its use in the following applications.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry (Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-BIN1 antibody (monoclonal), MA1005, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Figure 2. Western blot analysis of BIN1 using anti-BIN1 antibody (MA1005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysate,
Lane 2: mouse skeletal muscle tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BIN1 antigen affinity purified monoclonal antibody (Catalog # MA1005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BIN1 at approximately 65KD. The expected band size for BIN1 is at 65KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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Questions and answers from customers related to MA1005 Anti-BIN1 Antibody (Monoclonal, 99D)
7 Related Questions
Question
Does anti-BIN1 antibody (Monoclonal, 99D) MA1005 work on canine IHC with liver?
Verified Customer
Asked: 2020-01-10
Answer
Our lab technicians have not validated anti-BIN1 antibody (Monoclonal, 99D) MA1005 on canine. You can run a BLAST between canine and the immunogen sequence of anti-BIN1 antibody (Monoclonal, 99D) MA1005 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-10
Question
Is this MA1005 anti-BIN1 antibody (Monoclonal, 99D) reactive to the isotypes of BIN1?
Verified Customer
Asked: 2019-07-09
Answer
The immunogen of MA1005 anti-BIN1 antibody (Monoclonal, 99D) is Recombinant polypeptide containing amino acids 189-398 of human Bin1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
See below the WB image, lot number and protocol we used for leukemic t-cell using anti-BIN1 antibody (Monoclonal, 99D) MA1005. Please let me know if you require anything else.
Verified Customer
Asked: 2017-06-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-22
Question
I was wanting to use your anti-BIN1 antibody (Monoclonal, 99D) for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukemic t-cell identification?
Verified Customer
Asked: 2017-06-16
Answer
As indicated on the product datasheet, MA1005 anti-BIN1 antibody (Monoclonal, 99D) has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-16
Question
We are currently using anti-BIN1 antibody (Monoclonal, 99D) MA1005 for mouse tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
T. Moore
Asked: 2016-10-07
Answer
The anti-BIN1 antibody (Monoclonal, 99D) (MA1005) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-07
Question
I see that the anti-BIN1 antibody (Monoclonal, 99D) MA1005 works with WB, what is the protocol used to produce the result images on the product page?
H. Roberts
Asked: 2015-08-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-08-03
Question
Do you have a BSA free version of anti-BIN1 antibody (Monoclonal, 99D) MA1005 available?
S. Zhao
Asked: 2014-10-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-BIN1 antibody (Monoclonal, 99D) MA1005 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-16
