|Validated Species:||Human, Mouse, Rat|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-BIN1 Antibody (Monoclonal, 99D)|
|Description||Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-BIN1 Antibody (Monoclonal, 99D) 99D (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005)|
|Replacement Item||This antibody may replace the following items: sc-9360|sc-6320|sc-6319|sc-162325|sc-162326|sc-162327|sc-6319|sc-6320|sc-7015|sc-8357|sc-9360 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Recombinant polypeptide containing amino acids 189-398 of human Bin1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Myc box-dependent-interacting protein 1|
|Molecular Weight||64533 MW|
|Protein Function||May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. .|
|Tissue Specificity||Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed.|
|Sequence Similarities||Contains 1 BAR domain.|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Alternative Names||Myc box-dependent-interacting protein 1;Amphiphysin II;Amphiphysin-like protein;Bridging integrator 1;Bin1;Amph2, Amphl;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors| microbiology|interspecies interaction|host virus interaction| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors||
Background for Myc box-dependent-interacting protein 1
Anti-BIN1 Antibody (Monoclonal, 99D) Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate