Click image to see more details
Product Info Summary
| SKU: | MA1005 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-BIN1 Antibody (Monoclonal, 99D)
SKU/Catalog Number
MA1005
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BIN1 Antibody (Monoclonal, 99D) catalog # MA1005. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BIN1 Antibody (Monoclonal, 99D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
99D
Isotype
Mouse IgG2b
Immunogen
Recombinant polypeptide containing amino acids 189-398 of human Bin1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins
Reactive Species
MA1005 is reactive to Bin1 in Human, Mouse, Rat
Applications
MA1005 is guaranteed for IHC, ICC, WB Boster Guarantee
Background of Bin1
BIN1 (AMPH2) is a novel human gene product with features of a tumor suppressor protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry (Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat
Validation Images & Assay Conditions
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Anti-BIN1 antibody (monoclonal), MA1005, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Click image to see more details
Figure 2. Western blot analysis of BIN1 using anti-BIN1 antibody (MA1005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysate,
Lane 2: mouse skeletal muscle tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BIN1 antigen affinity purified monoclonal antibody (Catalog # MA1005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BIN1 at approximately 65KD. The expected band size for BIN1 is at 65KD.
Protein Target Info & Infographic
Gene/Protein Information For Bin1 (Source: Uniprot.Org, NCBI)
Uniprot ID
O08839
Gene ID
117028
Gene Name
Bin1
Full Name
Myc box-dependent-interacting protein 1
Weight
64.533kDa
Alternative Names
AMPH2; Amphiphysin II; Amphiphysin-like protein; AMPHLDKFZp547F068; box dependant MYC interacting protein 1; bridging integrator 1Box-dependent myc-interacting protein 1; MGC10367; myc box-dependent-interacting protein 1; SH3P9 Bin1|bridging integrator 1|myc box-dependent-interacting protein 1|Amph2|amphiphysin II|amphiphysin IIamph2|amphiphysin-like protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Bin1, check out the Bin1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Bin1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-BIN1 Antibody (Monoclonal, 99D) (MA1005)
No publications found for MA1005
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-BIN1 Antibody (Monoclonal, 99D)
Question
Does anti-BIN1 antibody (Monoclonal, 99D) MA1005 work on canine IHC with liver?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
Our lab technicians have not validated anti-BIN1 antibody (Monoclonal, 99D) MA1005 on canine. You can run a BLAST between canine and the immunogen sequence of anti-BIN1 antibody (Monoclonal, 99D) MA1005 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-10
Question
Is this MA1005 anti-BIN1 antibody (Monoclonal, 99D) reactive to the isotypes of BIN1?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of MA1005 anti-BIN1 antibody (Monoclonal, 99D) is Recombinant polypeptide containing amino acids 189-398 of human Bin1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
See below the WB image, lot number and protocol we used for leukemic t-cell using anti-BIN1 antibody (Monoclonal, 99D) MA1005. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-22
Question
I was wanting to use your anti-BIN1 antibody (Monoclonal, 99D) for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
As indicated on the product datasheet, MA1005 anti-BIN1 antibody (Monoclonal, 99D) has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-16
Question
We are currently using anti-BIN1 antibody (Monoclonal, 99D) MA1005 for mouse tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
T. Moore
Verified customer
Asked: 2016-10-07
Answer
The anti-BIN1 antibody (Monoclonal, 99D) (MA1005) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-07
Question
I see that the anti-BIN1 antibody (Monoclonal, 99D) MA1005 works with WB, what is the protocol used to produce the result images on the product page?
H. Roberts
Verified customer
Asked: 2015-08-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-08-03
Question
Do you have a BSA free version of anti-BIN1 antibody (Monoclonal, 99D) MA1005 available?
S. Zhao
Verified customer
Asked: 2014-10-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-BIN1 antibody (Monoclonal, 99D) MA1005 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-16
