SKU MA1005
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number 99D
Host Mouse
Ig Isotype Mouse IgG2b
Applications IHC, ICC, WB

Overview

Product Name Anti-BIN1 Antibody (Monoclonal, 99D)
SKU/Catalog Number MA1005
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-BIN1 Antibody (Monoclonal, 99D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005)
Host Mouse
Immunogen Recombinant polypeptide containing amino acids 189-398 of human Bin1.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry(Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Images And Assay Conditions

/antibody/ma1005 1 WB anti bin1 antibody monoclonal 99d.jpg

Anti-BIN1 antibody (monoclonal), MA1005, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O08839
Gene Name Bin1
Protein Name Myc box-dependent-interacting protein 1
Tissue Specificity Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed.
Alternative Names Myc box-dependent-interacting protein 1;Amphiphysin II;Amphiphysin-like protein;Bridging integrator 1;Bin1;Amph2, Amphl;
Subcellular Localization Cytoplasm . Nucleus .
Molecular Weight 64533 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. .
Background BIN1(AMPH2) is a novel human gene product with features of a tumor suppressor protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei.

Order Product (MA1005)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

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Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.