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| SKU | MA1005 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Clone Number | 99D |
| Host | Mouse |
| Ig Isotype | Mouse IgG2b |
| Applications | IHC, ICC, WB |
Overview
| Product Name | Anti-BIN1 Antibody (Monoclonal, 99D) |
|---|---|
| SKU/Catalog Number | MA1005 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins. |
| Cite This Product | Anti-BIN1 Antibody (Monoclonal, 99D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005) |
| Host | Mouse |
| Immunogen | Recombinant polypeptide containing amino acids 189-398 of human Bin1. |
| Reactivity | Human, Mouse, Rat |
Assay Details
Assay Dilutions Overview
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry(Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat
Immunohistochemistry(Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.Images And Assay Conditions
Anti-BIN1 antibody (monoclonal), MA1005, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | O08839 |
|---|---|
| Gene Name | Bin1 |
| Protein Name | Myc box-dependent-interacting protein 1 |
| Tissue Specificity | Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed. |
| Alternative Names | Myc box-dependent-interacting protein 1;Amphiphysin II;Amphiphysin-like protein;Bridging integrator 1;Bin1;Amph2, Amphl; |
| Subcellular Localization | Cytoplasm . Nucleus . |
| Molecular Weight | 64533 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. . |
|---|---|
| Background | BIN1(AMPH2) is a novel human gene product with features of a tumor suppressor protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei. |
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Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.