Overview
| Product Name |
Anti-BIN1 Antibody (Monoclonal, 99D)
See all Bin1 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
MA1005 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description |
Monoclonal clone# G2 antibody for AMPHIPHYSIN II/BIN1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-F. Reactive species: Human. AMPHIPHYSIN II/BIN1 information: Molecular Weight: 64533 MW; Subcellular Localization: Cytoplasm . Nucleus ; Tissue Specificity: Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed. |
| Cite This Product |
Anti-BIN1 Antibody (Monoclonal, 99D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005)
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Anti-BIN1 Antibody (Monoclonal, 99D) may replace the following items: sc 9360|sc 6320|sc 6319|sc 162325|sc 162326|sc 162327|sc 6319|sc 6320|sc 7015|sc 8357|sc 9360. |
Product Specs
| Host |
Mouse |
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
Recombinant polypeptide containing amino acids 189-398 of human Bin1. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For Bin1 (Source: Uniprot.org, NCBI)
| Uniprot Id | O08839 |
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| NCBI Gene Id | 117028 |
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| Species Of This Entry | Rat |
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| Gene Name | Bin1 |
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| Protein Name | Myc box-dependent-interacting protein 1 |
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| Alternative Names | Bin1|AMPH2; Amphiphysin II; Amphiphysin-like protein; AMPHLDKFZp547F068; box dependant MYC interacting protein 1; bridging integrator 1Box-dependent myc-interacting protein 1; MGC10367; myc box-dependent-interacting protein 1; SH3P9 |
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| Molecular Weight | 64533 |
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| Post Tranlational Modifications | Phosphorylation; Methylation; Cleavage; Biotinylation |
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| Gene Location | 18p12, on Chromasome 18, gene sequence: |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Bin1 (Source: Uniprot.org, NCBI)
| Protein Function | Is a key player in the control of plasma membrane curvature, and membrane shaping and remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (By similarity). Is a negative regulator of endocytosis (PubMed:9736607, PubMed:27760323). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (By similarity). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (PubMed:27760323). May be involved in the regulation of MYC activity and the control cell proliferation (By similarity). |
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| Tissue Specificity | Highly expressed in the brain and muscle. Isoform AMPH2-1 is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform AMPH2-2 is widely expressed. |
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| Related Diseases | Neoplasms; Malignant Neoplasms; Alzheimer's Disease; Cell Transformation, Neoplastic; Myopathy; Congenital Structural Myopathy; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Breast; Weakness; Mammary Neoplasms; Carcinoma; Congenital Myopathy (disorder); Dementia; Malignant Neoplasm Of Prostate; Neoplasm Metastasis; Metaplastic Cell Transformation; Liver Carcinoma; Neuroblastoma; Muscle Weakness; Tumor Progression |
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| Related Pathways | Endocytosis; Localization; Pathogenesis; Cell Death; Cell Proliferation; Cell Cycle; Dna Repair; Programmed Cell Death; Aging; Methylation; Cell Growth; Dna Methylation; Transport; Clathrin-mediated Endocytosis; Cytokinesis; Cell Motility; Cell Differentiation; Cell-cell Adhesion; Oncogenesis; Chromatin Remodeling |
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| Background | BIN1(AMPH2) is a novel human gene product with features of a tumor suppressor protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei. |
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| Scientific References | PMID: 9348539 by Wigge P., et al. Amphiphysin heterodimers: potential role in clathrin-mediated endocytosis.
PMID: 9280305 by McMahon H.T., et al. Clathrin interacts specifically with amphiphysin and is displaced by dynamin. |
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Our Boster Quality Guarantee for Anti-BIN1 Antibody (Monoclonal, 99D) covers its use in the following applications.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry(Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-BIN1 antibody (monoclonal), MA1005, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Figure 2. Western blot analysis of BIN1 using anti-BIN1 antibody (MA1005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysate,
Lane 2: mouse skeletal muscle tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BIN1 antigen affinity purified monoclonal antibody (Catalog # MA1005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BIN1 at approximately 65KD. The expected band size for BIN1 is at 65KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Bin1 primary antibodies,
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