SKU MA1005
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number 99D
Host Mouse
Ig Isotype Mouse IgG2b
Applications IHC, ICC, WB

Overview

Product Name Anti-BIN1 Antibody (Monoclonal, 99D)
SKU/Catalog Number MA1005
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.
Cite This Product Anti-BIN1 Antibody (Monoclonal, 99D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Form Lyophilized
Immunogen Recombinant polypeptide containing amino acids 189-398 of human Bin1.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry(Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Images And Assay Conditions

/antibody/ma1005 1 WB anti bin1 antibody monoclonal 99d.jpg

Anti-BIN1 antibody (monoclonal), MA1005, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate

/m/a/ma1005 2.jpg

Figure 2. Western blot analysis of BIN1 using anti-BIN1 antibody (MA1005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysate,
Lane 2: mouse skeletal muscle tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BIN1 antigen affinity purified monoclonal antibody (Catalog # MA1005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BIN1 at approximately 65KD. The expected band size for BIN1 is at 65KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O08839
Gene Name BIN1
Protein Name Myc box-dependent-interacting protein 1
Tissue Specificity Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed.
Alternative Names Myc box-dependent-interacting protein 1;Amphiphysin II;Amphiphysin-like protein;Bridging integrator 1;Bin1;Amph2, Amphl;
Subcellular Localization Cytoplasm . Nucleus .
Molecular Weight 64533 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. .
Research Areas Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics And Nuclear Signaling, Host Virus Interaction, Interspecies Interaction, Microbiology, Oncoproteins/Suppressors, Transcription, Tumor Suppressors

*You can search these to find other products in these research areas.
Background BIN1(AMPH2) is a novel human gene product with features of a tumor suppressor protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei.

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Order Product (MA1005)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships next business day.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.