Anti-BIN1 Antibody (Monoclonal, 99D)
Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.
Anti-BIN1 Antibody (Monoclonal, 99D) Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
|Product Name||Anti-BIN1 Antibody (Monoclonal, 99D)
See all Bin1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for AMPHIPHYSIN II/BIN1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-F. Reactive species: Human. AMPHIPHYSIN II/BIN1 information: Molecular Weight: 64533 MW; Subcellular Localization: Cytoplasm . Nucleus ; Tissue Specificity: Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed.|
|Cite This Product||Anti-BIN1 Antibody (Monoclonal, 99D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005)|
|Similar Products From
|Anti-BIN1 Antibody (Monoclonal, 99D) may replace the following items: sc 9360|sc 6320|sc 6319|sc 162325|sc 162326|sc 162327|sc 6319|sc 6320|sc 7015|sc 8357|sc 9360.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||Recombinant polypeptide containing amino acids 189-398 of human Bin1.|
|Cross Reactivity||No cross reactivity with other proteins|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Myc box-dependent-interacting protein 1|
|Subcellular Localization||Cytoplasm . Nucleus .|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Our Boster Quality Guarantee for Anti-BIN1 Antibody (Monoclonal, 99D) covers its use in the following applications.Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry(Frozen Section), 0.5μg/ml, Human, mouse, rat, -
Western blot, 0.25μg/ml, Human, mouse, rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysate,
Lane 2: mouse skeletal muscle tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BIN1 antigen affinity purified monoclonal antibody (Catalog # MA1005) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BIN1 at approximately 65KD. The expected band size for BIN1 is at 65KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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