|Application:||IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-BIN1 Antibody (Monoclonal, 99D)|
|Description||Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-BIN1 Antibody (Monoclonal, 99D) 99D (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1005)|
|Replacement Item||This antibody may replace the following items: sc-9360|sc-6320|sc-6319|sc-162325|sc-162326|sc-162327|sc-6319|sc-6320|sc-7015|sc-8357|sc-9360 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
|Immunogen||Recombinant polypeptide containing amino acids 189-398 of human Bin1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Myc box-dependent-interacting protein 1|
|Gene Full Name||Myc box-dependent-interacting protein 1|
|Protein Function||May be involved in regulation of synaptic vesicleendocytosis. May act as a tumor suppressor and inhibits malignantcell transformation..|
|Tissue Specificity||Isoform AMPH2-1 is expressed in brain,concentrated at nerve terminals. Isoform AMPH2-2 is widelyexpressed.|
|Sequence Similarities||Contains 1 BAR domain.|
|Subcellular Localization||Cytoplasm. Nucleus.|
|Uniprot ID||Bin1: O08839
|Alternative Names||Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Bridging integrator 1; Bin1; Amph2, Amphl;|
|Research Areas||cell biology|cell cycle|cell cycle inhibitors|microbiology|interspecies interaction|host virus interaction|epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors|cancer|oncoproteins/suppressors||
Background for Myc box-dependent-interacting protein 1
At Boster we strive to provide the best Anti-BIN1 Antibody (Monoclonal, 99D) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Application||Concentration*||Tested Species||Predicted Species||Antigen Retrieval|
|Immunocytochemistry||1μg/ml||Human, mouse, rat||-|
|Immunohistochemistry(Frozen Section)||0.5μg/ml||Human, mouse, rat||-|
|Western blot||0.25μg/ml||Human, mouse, rat||-|
Other applications have not been tested.
Optimal dilutions should be determined by end users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-BIN1 Antibody (Monoclonal, 99D) Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate