Anti-Blood Group Antigen A (CD173) ABO Monoclonal Antibody

ABO antibody

Boster Bio Anti-Blood Group Antigen A (CD173) ABO Monoclonal Antibody (Catalog # M00315). Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M00315
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: IHC

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Product Name

Anti-Blood Group Antigen A (CD173) ABO Monoclonal Antibody

View all ABO Antibodies

SKU/Catalog Number

M00315

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Blood Group Antigen A (CD173) ABO Monoclonal Antibody (Catalog # M00315). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Blood Group Antigen A (CD173) ABO Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00315)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 3-3A

Isotype

IgG1, kappa

Immunogen

Mucin isolated from an ovarian cyst fluid

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00315 is reactive to ABO in Human

Applications

M00315 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

40.934kDa

Background of ABO

This monoclonal antibody preferably reacts with determinants of chain A and H type 3(Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This monoclonal antibody is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For ABO (Source: Uniprot.org, NCBI)

Gene Name

ABO

Full Name

Histo-blood group ABO system transferase

Weight

40.934kDa

Superfamily

glycosyltransferase 6 family

Alternative Names

A transferase; A3GALNT; A3GALT1; ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase); ABO glycosyltransferase; B transferase; B(A) alpha-1,3-galactosyltransferase; EC 2.4.1.37; EC 2.4.1.40; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; GTB; Histo-blood group A transferase; histo-blood group A2 transferase; histo-blood group AB ABO A3GALNT, A3GALT1, GTB, NAGAT ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase histo-blood group ABO system transferase|ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)|B(A) alpha-1,3-galactosyltransferase|histo-blood group A2 transferase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ABO, check out the ABO Infographic

ABO infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ABO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Blood Group Antigen A (CD173) ABO Monoclonal Antibody

Question

We are currently using anti-Blood Group antigen A (CD173) Monoclonal antibody M00315 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

The anti-Blood Group antigen A (CD173) Monoclonal antibody (M00315) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-05

Size

Total: $468

SKU:M00315

Backordered.

Lead time for this item is typically 6-8 days

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M00315
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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