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SKU:PA1904
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-BOC Antibody
Description Rabbit IgG polyclonal antibody for Brother of CDO(BOC) detection. Tested with WB in Mouse;Rat.
Cite This Product Anti-BOC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1904)
Replacement Item This antibody may replace the following items: sc-368211|sc-51258-R from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOC(1002-1020aa DDSTHQLLQPQDCCHLQKQ), identical to the related rat sequence.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Boc
Protein Name Brother of CDO
Molecular Weight 121331 MW
Protein Function Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells.
Tissue Specificity Highly expressed in embryonic somites, limb buds, dermomyotomes and in the neural tube. .
Sequence Similarities Contains 3 fibronectin type-III domains.
Subcellular Localization Membrane ; Single-pass type I membrane protein .
Uniprot ID Q6AZB0
Alternative Names Brother of CDO;Protein BOC;Boc;
Research Areas |stem cells|signaling pathways|hedgehog|surface molecules| stem cells|mesenchymal stem cells|myogenesis| developmental biology|organogenesis|skeletal development|muscle|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Brother of CDO

BOC(Brother Of CDO) also known as BROTHER OF CDON and BOC are cell surface receptors of the immunoglobulin(Ig)/fibronectin type III repeat family involved in myogenic differentiation. CDON and BOC are coexpressed during development, form complexes with each other in a cis fashion, and are related to each other in their ectodomains, but each has a unique long cytoplasmic tail. The domain structure of BOC is similar to that of CDON, and the 2 proteins share 38 to 80% amino acid identity in the individual extracellular domains. The International Radiation Hybrid Mapping Consortium mapped the BOC gene to chromosome 3. In contrast with CDON, the intracellular region of BOC was not essential for its ability to positively regulate differentiation.BOC is dependent on CDON for activity and that CDON-BOC complexes function to regulate myogenic differentiation. Wegorzewska et al.(2003) proposed that CDO and BOC play a role in the inverse relationship between differentiation and transformation of cells in the skeletal muscle lineage.BOC is expressed by commissural neurons, and targeted disruption of BOC in mouse resulted in the misguidance of commissural axons toward the floor plate.

Anti-BOC Antibody Images

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Anti-BOC Antibody
Anti- BOC antibody, PA1904, Western blotting
All lanes: Anti BOC (PA1904) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 121KD
Observed bind size: 121KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.