Anti-BORG3 CDC42EP5 Antibody

Cdc42ep5 antibody

Boster Bio Anti-BORG3 CDC42EP5 Antibody (Catalog # A14184). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A14184
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-BORG3 CDC42EP5 Antibody

View all Cdc42ep5 Antibodies

SKU/Catalog Number

A14184

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-BORG3 CDC42EP5 Antibody (Catalog # A14184). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BORG3 CDC42EP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14184)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human BORG3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14184 is reactive to Cdc42ep5 in Human, Mouse

Applications

A14184 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

15.545kDa

Background of Cdc42ep5

Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Inhibits MAPK8 independently of CDC42 binding. Controls septin organization and this effect is negatively regulated by CDC42 By similarity.

The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Cdc42ep5 (Source: Uniprot.org, NCBI)

Gene Name

Cdc42ep5

Full Name

Cdc42 effector protein 5

Weight

15.545kDa

Superfamily

BORG/CEP family

Alternative Names

Binder of Rho GTPases 3; Borg3; CDC42 effector protein (Rho GTPase binding) 5,2010007O02Rik; cdc42 effector protein 5,1700027J19Rik; CEP5MGC21945; MGC71153

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cdc42ep5, check out the Cdc42ep5 Infographic

Cdc42ep5 infographic

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In this infographic, you will see the following information for Cdc42ep5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-BORG3 CDC42EP5 Antibody

Question

Can you help my question with product A14184, anti-BORG3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14184 anti-BORG3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-18

Question

I see that the anti-BORG3 antibody A14184 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-09-04

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-09-04

Question

Would A14184 anti-BORG3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Huang

Verified customer

Asked: 2018-02-22

Answer

It shows on the product datasheet, A14184 anti-BORG3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-22

Question

I am interested in to test anti-BORG3 antibody A14184 on human colonic mucosa for research purposes, then I may be interested in using anti-BORG3 antibody A14184 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-12-26

Answer

The products we sell, including anti-BORG3 antibody A14184, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-12-26

Question

Is a blocking peptide available for product anti-BORG3 antibody (A14184)?

G. Brown

Verified customer

Asked: 2013-05-17

Answer

We do provide the blocking peptide for product anti-BORG3 antibody (A14184). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-05-17

Order DetailsPrice
A14184-100uL

100uL

$360

Free Secondary Antibody

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A14184
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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