Product Info Summary
SKU: | A14184 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-BORG3 CDC42EP5 Antibody
SKU/Catalog Number
A14184
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-BORG3 CDC42EP5 Antibody (Catalog # A14184). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BORG3 CDC42EP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14184)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human BORG3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14184 is reactive to Cdc42ep5 in Human, Mouse
Applications
A14184 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
15.545kDa
Background of Cdc42ep5
Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Inhibits MAPK8 independently of CDC42 binding. Controls septin organization and this effect is negatively regulated by CDC42 By similarity.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using BORG3 antibody A14184.
Protein Target Info & Infographic
Gene/Protein Information For Cdc42ep5 (Source: Uniprot.org, NCBI)
Gene Name
Cdc42ep5
Full Name
Cdc42 effector protein 5
Weight
15.545kDa
Superfamily
BORG/CEP family
Alternative Names
Binder of Rho GTPases 3; Borg3; CDC42 effector protein (Rho GTPase binding) 5,2010007O02Rik; cdc42 effector protein 5,1700027J19Rik; CEP5MGC21945; MGC71153
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cdc42ep5, check out the Cdc42ep5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cdc42ep5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BORG3 CDC42EP5 Antibody (A14184)
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No publications found for A14184
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-BORG3 CDC42EP5 Antibody
Question
Can you help my question with product A14184, anti-BORG3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14184 anti-BORG3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-18
Question
I see that the anti-BORG3 antibody A14184 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-04
Question
Would A14184 anti-BORG3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Huang
Verified customer
Asked: 2018-02-22
Answer
It shows on the product datasheet, A14184 anti-BORG3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-22
Question
I am interested in to test anti-BORG3 antibody A14184 on human colonic mucosa for research purposes, then I may be interested in using anti-BORG3 antibody A14184 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
The products we sell, including anti-BORG3 antibody A14184, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-26
Question
Is a blocking peptide available for product anti-BORG3 antibody (A14184)?
G. Brown
Verified customer
Asked: 2013-05-17
Answer
We do provide the blocking peptide for product anti-BORG3 antibody (A14184). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-17