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SKU:PA1318
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-c-Fos Antibody
SKU/Catalog Number PA1318
Description Rabbit IgG polyclonal antibody for Proto-oncogene c-Fos(FOS) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-c-Fos Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1318)
Replacement Item This antibody may replace the following items: sc-13017-X|sc-130181|sc-166102-X|sc-166940|sc-166940-X|sc-171-X|sc-183-X|sc-22794|sc-22794-X|sc-253|sc-253-G|sc-253-X|sc-271243|sc-271243-X|sc-271657|sc-271657-X|sc-28213|sc-28213-X|sc-28310|sc-28310-X|sc-376148|sc-376148-X|sc-390035|sc-390035-X|sc-398595|sc-398595-X|sc-413|sc-413-X|sc-447|sc-447-X|sc-48-G|sc-48-X|sc-48424|sc-48868-X|sc-48869-X|sc-52|sc-52-G|sc-52-X|sc-604-X|sc-605-X|sc-69272-X|sc-69273-X|sc-7202|sc-7202-X|sc-7203-X|sc-8013-X|sc-8047|sc-8047-X|sc-81209|sc-81485 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human c-Fos(170-187aa DQLEDEKSALQTEIANLL), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name FOS
Protein Name Proto-oncogene c-Fos
Molecular Weight 40695 MW
Protein Function Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. .
Sequence Similarities Belongs to the bZIP family. Fos subfamily.
Subcellular Localization Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.
Uniprot ID P01100
Alternative Names Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7;FOS;G0S7;
Research Areas |epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| epigenetics and nuclear signaling|cancer susceptibility|proto-oncogenes| cancer|oncoproteins/suppressors|oncoproteins|transcription factors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Proto-oncogene c-Fos

The human oncogene c-fos is cellular homolog of the transforming gene of Finkel-Biskis-Jinkins(FBJ) murine osteosarcoma virus which was mapped to a single human chromosome. c-Fos is encoded by the FOS gene. FOS was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton. FOS is also a major component of the activator protein-1(AP-1) transcription factor complex, which includes members of the JUN family. c-fos is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. Using transgenic and knockout mice, Grigoriadis et al.(1995) established a unique role for the proto-oncogene and nuclear transcription factor, Fos, in regulating the differentiation and activity of specific bone cell populations, both during normal development and in bone disease.

Anti-c-Fos Antibody Images

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Anti-c-Fos Antibody
Anti-c-Fos antibody, PA1318, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
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Publications

Liu W, Peng Qx, Lin Xl, Luo Ch, Jiang Mj, Mo Zx, Yung Kk. Fitoterapia. 2014 Jan;92:16-22. Doi: 10.1016/J.Fitote.2013.10.002. Epub 2013 Oct 17. Effect Of Rhynchophylline On The Expression Of P-Creb And Sc-Fos In Triatum And Hippocampal Ca1 Area Of ...
Zhang H, Li L, Xu Gy, Mei Yw, Zhang Jj, Murong Sx, Sun Sg, Tong Et. J Huazhong Univ Sci Technolog Med Sci. 2014 Jun;34(3):354-8. Doi: 10.1007/S11596-014-1282-4. Epub 2014 Jun 18. Changes Of C-Fos, Malondialdehyde And Lactate In Brain Tissue After ...
Zhang S, He B, Goldstein S, Ge J, Wang Z, Ruiz G. Virol J. 2010 Oct 27;7:285. Doi: 10.1186/1743-422X-7-285. The Expression And Significance Of Proto-Oncogene C-Fos In Viral Myocarditis.
Zhang S, Zhang M, Goldstein S, Li Y, Ge J, He B, Ruiz G. Cell Biochem Biophys. 2013 Mar;65(2):249-55. Doi: 10.1007/S12013-012-9428-0. The Effect Of C-Fos On Acute Myocardial Infarction And The Significance Of Metoprolol Intervention In A Rat Model.
Lou Jl, Jiang Mn, Li C, Zhou Q, He X, Lei Hy, Li J, Jia Yj. J Ethnopharmacol. 2010 Mar 2;128(1):131-8. Doi: 10.1016/J.Jep.2009.12.038. Epub 2010 Jan 6. Herb Medicine Gan-Fu-Kang Attenuates Liver Injury In A Rat Fibrotic Model.
Peng S, Zhang Y, Ren B, Zhang J, Wang H. Mol Biol Rep. 2011 Apr;38(4):2401-7. Doi: 10.1007/S11033-010-0374-X. Epub 2010 Nov 16. Effect Of Ketamine Administration On Memory Consolidation, P-Creb And C-Fos Expression In The Hippocampal Slices Of Min...
Li R, Liang T, Chen Z, Zhang S, Lin X, Huang R. Gene. 2013 Sep 15;527(1):115-22. Doi: 10.1016/J.Gene.2013.05.072. Epub 2013 Jun 14. L-Dopa Methyl Ester Attenuates Amblyopia-Induced Neuronal Injury In Visual Cortex Of Amblyopic Cat.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to c fos antibody, ap 1 antibody, fos antibody