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SKU:MA1028
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:9E10
Application:IHC-P, ICC, WB
Price: $200.00
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Overview

Product Name Anti-c-Myc Antibody (Monoclonal, 9E10)
SKU/Catalog Number MA1028
Description Mouse IgG monoclonal antibody for c-Myc, v-myc myelocytomatosis viral oncogene homolog (avian) (MYC) detection. Tested with WB, IHC-P, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-c-Myc Antibody (Monoclonal, 9E10) 9E10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1028)
Replacement Item This antibody may replace the following items: sc-28207 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Predicted Species Monkey, Rabbit

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number 9E10
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Myc
Protein Name Myc proto-oncogene protein
Molecular Weight 48898 MW
Protein Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. .
Sequence Similarities Contains 1 bHLH (basic helix-loop-helix) domain.
Subcellular Localization Nucleus, nucleoplasm . Nucleus, nucleolus .
Uniprot ID P09416
Alternative Names Myc proto-oncogene protein;Proto-oncogene c-Myc;Transcription factor p64;Myc;
Research Areas |epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| stem cells|signaling pathways|tgf beta|nuclear| epigenetics and nuclear signaling|transcription factors| cancer|cell cycle|cell differentiation|oncoproteins/suppressors|oncoproteins|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Myc proto-oncogene protein

C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Anti-c-Myc Antibody (Monoclonal, 9E10) Images

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Anti-c-Myc Antibody (Monoclonal, 9E10)
Anti-c-Myc antibody (monoclonal), MA1028, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
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Publications

Wang Ph, Gu Zh, Wan Dh, Zhu Wb, Qiu W, Weng Sp, Yu Xq, He Jg. Plos One. 2013;8(2):E52088. Doi: 10.1371/Journal.Pone.0052088. Epub 2013 Feb 6. Litopenaeus Vannamei Sterile-Alpha And Armadillo Motif Containing Protein (Lvsarm) Is Involved In Regulat...
Long Xb, Sun Gb, Hu S, Liang Gt, Wang N, Zhang Xh, Cao Pp, Zhen Ht, Cui Yh, Liu Z. Oncol Rep. 2009 Nov;22(5):1189-95. Let-7A Microrna Functions As A Potential Tumor Suppressor In Human Laryngeal Cancer.
Liu B, Liu F, Bai L, Li Y, Yang G. Mol Biol Rep. 2010 Mar;37(3):1671-6. Doi: 10.1007/S11033-009-9858-Y. Epub 2009 Oct 13. Molecular Cloning, Expression And Subcellular Distribution Of An Alternative Splice Variant Of The Porcine Sirt2 Gene.
Zhang K, Lu Y, Yang P, Li C, Sun H, Tao D, Liu Y, Zhang S, Ma Y. Plos One. 2012;7(7):E41973. Doi: 10.1371/Journal.Pone.0041973. Epub 2012 Jul 27. Hili Inhibits Tgf-?? Signaling By Interacting With Hsp90 And Promoting T??r Degradation.
Hao Hp, Wen Lb, Li Jr, Wang Y, Ni B, Wang R, Wang X, Sun Mx, Fan Hj, Mao X. Antiviral Res. 2015 May;117:99-109. Doi: 10.1016/J.Antiviral.2015.02.010. Epub 2015 Mar 5. Licl Inhibits Prrsv Infection By Enhancing Wnt/??-Catenin Pathway And Suppressing...
Wang H, Huo N, Li F, Fu S, Xue Y, Yang T, Wen X, Ding Y, Duan X. Mol Cell Biochem. 2010 Sep;342(1-2):191-9. Doi: 10.1007/S11010-010-0483-9. Epub 2010 May 16. Osteogenic Role Of Endosomal Chloride Channels In Mc3T3-E1 Cells.
Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J, Lu J. Oncol Rep. 2013 Jun;29(6):2291-6. Doi: 10.3892/Or.2013.2390. Epub 2013 Apr 8. Leptin Induces Cell Proliferation And Reduces Cell Apoptosis By Activating C-Myc In Cervical Cancer.
Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to c myc antibody, myc antibody, c myc tag antibody