Data & Images
|Product Name||Anti-complement C5a Antibody|
|Description||Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB, IHC-P in Mouse;Rat.|
|Cite This Product||Anti-complement C5a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2308)|
|Replacement Item||This antibody may replace the following items: sc-70476|sc-52637|sc-52636|sc-52635|sc-58934|sc-58933 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of rat C5a(1-18aa DLQLLHQKVEEQAAKYKH), different from the related mouse sequence by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Complement C5|
|Molecular Weight||8981 MW|
|Protein Function||Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. .|
|Alternative Names||Complement C5;C5a anaphylatoxin;C5;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| epigenetics and nuclear signaling|hlh| immunology|innate immunity|mast cells|chip'ing antibodies||
Background for Complement C5
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-complement C5a Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-complement C5a Antibody Images
Click the images to enlarge.
IHC(P): Mouse Spleen Tissue
IHC(P): Rat Liver Tissue
IHC(P): Rat Spleen Tissue
All lanes: Anti-C5a(PA2308) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Predicted bind size: 115KD
Observed bind size: 115KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,