Product Info Summary
SKU: | A04165-1 |
---|---|
Size: | 100μl |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-CABP CABP1 Antibody
SKU/Catalog Number
A04165-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CABP CABP1 Antibody catalog # A04165-1. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CABP CABP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04165-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
5G7
Isotype
IgG
Immunogen
Synthetic Peptide
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04165-1 is reactive to CABP1 in Mouse, Rat
Applications
A04165-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.838kDa
Background of CABP1
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of 1) Mouse Brain Tissue, 2) Rat Brain Tissue with CABP Rabbit Polyclonal antibody diluted at 1:2000.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain Tissue using CABP Rabbit Polyclonal antibody diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For CABP1 (Source: Uniprot.org, NCBI)
Gene Name
CABP1
Full Name
Calcium-binding protein 1
Weight
39.838kDa
Alternative Names
CaBP1; CALBRAIN; calcium binding protein 1; calcium binding protein 5; calcium-binding protein 1; caldendrin; HCALB_BR CABP1 CALBRAIN, HCALB_BR calcium binding protein 1 calcium-binding protein 1|calcium binding protein 5|caldendrin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CABP1, check out the CABP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CABP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CABP CABP1 Antibody (A04165-1)
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No publications found for A04165-1
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-CABP CABP1 Antibody
Question
I was wanting to use your anti-CABP antibody for IHC for rat anterior cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat anterior cingulate cortex identification?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
You can see on the product datasheet, A04165-1 anti-CABP antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-CABP antibody A04165-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-14
Question
Here is the WB image, lot number and protocol we used for anterior cingulate cortex using anti-CABP antibody A04165-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-16
Question
We are currently using anti-CABP antibody A04165-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The anti-CABP antibody (A04165-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-01
Question
Would anti-CABP antibody A04165-1 work on pig WB with retina?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Our lab technicians have not validated anti-CABP antibody A04165-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CABP antibody A04165-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig retina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-24
Question
Is there a BSA free version of anti-CABP antibody A04165-1 available?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CABP antibody A04165-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-04
Question
Will A04165-1 anti-CABP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
You can see on the product datasheet, A04165-1 anti-CABP antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-31
Question
Is this A04165-1 anti-CABP antibody reactive to the isotypes of CABP1?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
The immunogen of A04165-1 anti-CABP antibody is Synthetic Peptide. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-06
Question
I would like to test anti-CABP antibody A04165-1 on rat anterior cingulate cortex for research purposes, then I may be interested in using anti-CABP antibody A04165-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
The products we sell, including anti-CABP antibody A04165-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-11
Question
I see that the anti-CABP antibody A04165-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-16
Question
Would anti-CABP antibody A04165-1 work for IHC with anterior cingulate cortex?
Verified Customer
Verified customer
Asked: 2017-05-19
Answer
According to the expression profile of anterior cingulate cortex, CABP1 is highly expressed in anterior cingulate cortex. So, it is likely that anti-CABP antibody A04165-1 will work for IHC with anterior cingulate cortex.
Boster Scientific Support
Answered: 2017-05-19
Question
Is a blocking peptide available for product anti-CABP antibody (A04165-1)?
A. Collins
Verified customer
Asked: 2013-12-13
Answer
We do provide the blocking peptide for product anti-CABP antibody (A04165-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-12-13
Question
Can you help my question with product A04165-1, anti-CABP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Kulkarni
Verified customer
Asked: 2013-10-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04165-1 anti-CABP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-10-04