SKU MA1007
Size 100μg/vial
Reactivity Bovine, Human, Mouse, Rat
Clonality Monoclonal
Clone Number CB-955
Host Mouse
Ig Isotype Mouse IgG1
Applications IF, IHC-P, WB

Overview

Product Name Anti-Calbindin-D Antibody (Monoclonal, CB-955)
SKU/Catalog Number MA1007
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Calbindin-D, calbindin 1, 28kDa (CALB1) detection. Tested with WB, IHC-P, IF in Human;mouse;rat;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Calbindin-D Antibody (Monoclonal, CB-955) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1007)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Form Lyophilized
Immunogen Bovine kidney calbindin-D
Reactivity Bovine, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, bovine, mouse, rat, By Heat
Western blot, 0.5-1μg/ml, Human, bovine, mouse, rat
Immunofluorescence, 2μg/ml, Mouse

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1007 1 WB anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate

/antibody/ma1007 2 IHC anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Mouse Kidney Tissue

/antibody/ma1007 3 IHC anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Rat Kidney Tissue

/antibody/ma1007 4 IHC anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/antibody/ma1007 5 IHC anti calbindin d antibody monoclonal cb 955.jpg

Anti-Calbindin-D antibody (monoclonal), MA1007, IHC(P)
IHC(P): Human Mammary Cancer Tissue

/m/a/ma1007 6.jpg

Figure 6. Western blot analysis of Calbindin-D using anti-Calbindin-D antibody (MA1007).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates
Lane 2: rat kidney tissue lysates
Lane 3: mouse brain tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calbindin-D antigen affinity purified monoclonal antibody (Catalog # MA1007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calbindin-D at approximately 28KD. The expected band size for Calbindin-D is at 28KD.

/m/a/ma1007 7.jpg

Figure 7. IF analysis of Calbindin-D using anti- Calbindin-D antibody (MA1007)
Calbindin-D was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti- Calbindin-D Antibody (MA1007) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P07171
Gene Name CALB1
Protein Name Calbindin
Alternative Names Calbindin;Calbindin D28;D-28K;Spot 35 protein;Vitamin D-dependent calcium-binding protein, avian-type;Calb1;
Molecular Weight 29994 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Research Areas Calcium Binding Proteins, Calcium Signaling, Cell Type Marker, Neuron Marker, Neuroscience, Neurotransmission

*You can search these to find other products in these research areas.
Background The human calbindin D28k(CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al.(1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.

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Order Product (MA1007)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships next business day.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.